LOCUS       sequence-139692-         850 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  template for N-terminal 3x FLAG tag, constitutive Blasticidin 
            resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 850)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 850)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pRB34
FEATURES             Location/Qualifiers
     source          1..850
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    509..542
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 tcaaaagaga cgtcttttgt taagaatgct gaggaacttg caaagcaaaa aatggatgct
       61 attaaccctg aactttcttc aaaatttaaa tttttaataa aattcctgtc tcagtttcct
      121 gaagcttgct ctaaacctcg ttcaaaaaaa atgcagaata aagttggtca agaggaacat
      181 attgaatatt tagctcgtag ttttcatgag agtcgattgc caagaaaacc cacgccacct
      241 acaacggttc ctgatgaggt ggttagcata gttcttaata taagttttaa tatacagcct
      301 gaaaatcttg agagaataaa agaagaacat cgattttcca tggcagctga gaatattgta
      361 ggagatcttc tagaaagata ctagtaccgc cgcttggagc agctggagag gtacccttgt
      421 cgtcgtcctt gtagtcgatg tcgtggtcct tgtagtcgcc gtcgtggtcc ttgtagtcga
      481 attcaccggt ggatcccatt ttggattaga agttcctatt ctctagaaag tataggaact
      541 tccctaggtt tgtgttaaaa tattttgttg ttgattatat taaaataaac ttgattcact
      601 tagccgttag ctagcactgt ttgatgattg tcgactggcg gtccagcatt cttatagagc
      661 tttcactgct ttgcgcacca aaaaaatcaa ttcctgttat cagggcgtgt gcaaatcgct
      721 tatcatcgcc gggcagaaaa agcattatgc ttttgcgcac gccgatcgac ttgcacacgg
      781 ctttcttatc ttatcgggca ccacggccca cagtttcgat tggactggat cgggtataaa
      841 gaggaggatt
//
