LOCUS       sequence-139695-         863 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  template for N-terminal 1x Strep tag, constitutive Blasticidin 
            resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 863)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 863)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pRB32
FEATURES             Location/Qualifiers
     source          1..863
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(12..44)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             314..712
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
ORIGIN
        1 ttcagcaaga taagttccta tactttctag agaataggaa cttccatatg ctgttggaat
       61 atactattca acctacaaaa ataacgttaa acaacactac tttatatttg atatgaatgg
      121 ccacaccttt tatgccataa aacatattgt aagagaatac cactcttttt attccttctt
      181 tccttcttgt acgttttttg ctgtgagtag gtcgtggtgc tggtgttgca gttgaaataa
      241 cttaaaatat aaatcataaa actcaaacat aaacttgact atttatttat ttattaagaa
      301 aggaggtacc ggtatggcca agcctttgtc tcaagaagaa tccaccctca ttgaaagagc
      361 aacggctaca atcaacagca tccccatctc tgaagactac agcgtcgcca gcgcagctct
      421 ctctagcgac ggccgcatct tcactggtgt caatgtatat cattttactg ggggaccttg
      481 cgcagaactc gtggtgctgg gcactgctgc tgctgcggca gctggcaacc tgacttgtat
      541 cgtcgcgatc ggaaatgaga acaggggcat cttgagcccc tgcggacggt gccgacaggt
      601 tcttctcgat ctgcatcctg ggatcaaagc catagtgaag gacagtgatg gacagccgac
      661 ggcagttggg attcgtgaat tgctgccctc tggttatgtg tgggagggct agaaattatt
      721 aaaaataaaa ctttaaaaat aaatttgttt ctaacatatg cctaggcttg ttgatttgtt
      781 ttttgtttat ttactgcggt aattcctagt cttagaagct tattgaactt gttgatgcct
      841 tctggttggg aaatcctcct ctt
//
