LOCUS       sequence-139696-         809 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  template for N-terminal 1x Strep tag, constitutive Blasticidin 
            resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 809)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 809)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pRB32
FEATURES             Location/Qualifiers
     source          1..809
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(431..454)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    479..512
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 atgtcaaaag agacgtcttt tgttaagaat gctgaggaac ttgcaaagca aaaaatggat
       61 gctattaacc ctgaactttc ttcaaaattt aaatttttaa taaaattcct gtctcagttt
      121 cctgaagctt gctctaaacc tcgttcaaaa aaaatgcaga ataaagttgg tcaagaggaa
      181 catattgaat atttagctcg tagttttcat gagagtcgat tgccaagaaa acccacgcca
      241 cctacaacgg ttcctgatga ggtggttagc atagttctta atataagttt taatatacag
      301 cctgaaaatc ttgagagaat aaaagaagaa catcgatttt ccatggcagc tgagaatatt
      361 gtaggagatc ttctagaaag atactagtac cgccgcttgg agcagctgga gaggtaccga
      421 attcaccggt tttttcgaac tgcgggtggc tccaagcgct ggatcccatt ttggattaga
      481 agttcctatt ctctagaaag tataggaact tccctaggtt tgtgttaaaa tattttgttg
      541 ttgattatat taaaataaac ttgattcact tagccgttag ctagcactgt ttgatgattg
      601 tcgactggcg gtccagcatt cttatagagc tttcactgct ttgcgcacca aaaaaatcaa
      661 ttcctgttat cagggcgtgt gcaaatcgct tatcatcgcc gggcagaaaa agcattatgc
      721 ttttgcgcac gccgatcgac ttgcacacgg ctttcttatc ttatcgggca ccacggccca
      781 cagtttcgat tggactggat cgggtataa
//
