LOCUS       sequence-139697-         765 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  template for N-terminal Strep-8xHis-Strep-TEV site tag, constitutive
            Blasticidin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 765)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 765)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pRB38
FEATURES             Location/Qualifiers
     source          1..765
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(15..47)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             317..715
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
ORIGIN
        1 tttttcagca agataagttc ctatactttc tagagaatag gaacttccat atgctgttgg
       61 aatatactat tcaacctaca aaaataacgt taaacaacac tactttatat ttgatatgaa
      121 tggccacacc ttttatgcca taaaacatat tgtaagagaa taccactctt tttattcctt
      181 ctttccttct tgtacgtttt ttgctgtgag taggtcgtgg tgctggtgtt gcagttgaaa
      241 taacttaaaa tataaatcat aaaactcaaa cataaacttg actatttatt tatttattaa
      301 gaaaggaggt accggtatgg ccaagccttt gtctcaagaa gaatccaccc tcattgaaag
      361 agcaacggct acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc
      421 tctctctagc gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc
      481 ttgcgcagaa ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg
      541 tatcgtcgcg atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca
      601 ggttcttctc gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc
      661 gacggcagtt gggattcgtg aattgctgcc ctctggttat gtgtgggagg gctagaaatt
      721 attaaaaata aaactttaaa aataaatttg tttctaacat atgcc
//
