LOCUS       sequence-139698-         791 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  template for N-terminal Strep-8xHis-Strep-TEV site tag, constitutive
            Blasticidin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 791)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 791)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pRB38
FEATURES             Location/Qualifiers
     source          1..791
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(426..446)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(459..482)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(489..512)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(519..542)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    567..600
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 catgtcaaaa gagacgtctt ttgttaagaa tgctgaggaa cttgcaaagc aaaaaatgga
       61 tgctattaac cctgaacttt cttcaaaatt taaattttta ataaaattcc tgtctcagtt
      121 tcctgaagct tgctctaaac ctcgttcaaa aaaaatgcag aataaagttg gtcaagagga
      181 acatattgaa tatttagctc gtagttttca tgagagtcga ttgccaagaa aacccacgcc
      241 acctacaacg gttcctgatg aggtggttag catagttctt aatataagtt ttaatataca
      301 gcctgaaaat cttgagagaa taaaagaaga acatcgattt tccatggcag ctgagaatat
      361 tgtaggagat cttctagaaa gatactagta ccgccgcttg gagcagctgg agaggtaccg
      421 aattcgccct gaaaatacag gttttcgaat tcaccggttt tttcgaactg cgggtggctc
      481 cacgatccgt gatgatggtg atggtggtga tgtccacctt tctcgaactg cgggtggctc
      541 caagcgctgg atcccatttt ggattagaag ttcctattct ctagaaagta taggaacttc
      601 cctaggtttg tgttaaaata ttttgttgtt gattatatta aaataaactt gattcactta
      661 gccgttagct agcactgttt gatgattgtc gactggcggt ccagcattct tatagagctt
      721 tcactgcttt gcgcaccaaa aaaatcaatt cctgttatca gggcgtgtgc aaatcgctta
      781 tcatcgccgg g
//
