LOCUS       sequence-139699-         826 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  template for N-terminal eGFP tag, constitutive Blasticidin 
            resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 826)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 826)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pRB40
FEATURES             Location/Qualifiers
     source          1..826
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(5..37)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
     CDS             307..705
                     /codon_start=1
                     /gene="Aspergillus terreus BSD"
                     /product="blasticidin S deaminase"
                     /label=BSD
                     /note="confers resistance to blasticidin"
                     /translation="MAKPLSQEESTLIERATATINSIPISEDYSVASAALSSDGRIFTG
                     VNVYHFTGGPCAELVVLGTAAAAAAGNLTCIVAIGNENRGILSPCGRCRQVLLDLHPGI
                     KAIVKDSDGQPTAVGIRELLPSGYVWEG"
ORIGIN
        1 agataagttc ctatactttc tagagaatag gaacttccat atgctgttgg aatatactat
       61 tcaacctaca aaaataacgt taaacaacac tactttatat ttgatatgaa tggccacacc
      121 ttttatgcca taaaacatat tgtaagagaa taccactctt tttattcctt ctttccttct
      181 tgtacgtttt ttgctgtgag taggtcgtgg tgctggtgtt gcagttgaaa taacttaaaa
      241 tataaatcat aaaactcaaa cataaacttg actatttatt tatttattaa gaaaggaggt
      301 accggtatgg ccaagccttt gtctcaagaa gaatccaccc tcattgaaag agcaacggct
      361 acaatcaaca gcatccccat ctctgaagac tacagcgtcg ccagcgcagc tctctctagc
      421 gacggccgca tcttcactgg tgtcaatgta tatcatttta ctgggggacc ttgcgcagaa
      481 ctcgtggtgc tgggcactgc tgctgctgcg gcagctggca acctgacttg tatcgtcgcg
      541 atcggaaatg agaacagggg catcttgagc ccctgcggac ggtgccgaca ggttcttctc
      601 gatctgcatc ctgggatcaa agccatagtg aaggacagtg atggacagcc gacggcagtt
      661 gggattcgtg aattgctgcc ctctggttat gtgtgggagg gctagaaatt attaaaaata
      721 aaactttaaa aataaatttg tttctaacat atgcctaggc ttgttgattt gttttttgtt
      781 tatttactgc ggtaattcct agtcttagaa gcttattgaa cttgtt
//
