LOCUS       sequence-139701-         799 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  template base vector for N-terminal tagging, constitutive Puromycin 
            resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 799)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 799)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pRB35
FEATURES             Location/Qualifiers
     source          1..799
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(22..54)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 gctcgagttt ttcagcaaga taagttccta tactttctag agaataggaa cttccatatg
       61 ggcgcgccct gttggaatat actattcaac ctacaaaaat aacgttaaac aacactactt
      121 tatatttgat atgaatggcc acacctttta tgccataaaa catattgtaa gagaatacca
      181 ctctttttat tccttctttc cttcttgtac gttttttgct gtgagtaggt cgtggtgctg
      241 gtgttgcagt tgaaataact taaaatataa atcataaaac tcaaacataa acttgactat
      301 ttatttattt attaagaaag gaggtaccgg tggcgcgcca tgaccgagta caagcctaca
      361 gtgcgactgg ccacacgcga tgacgttccc agagccgttc ggacgttggc tgccgcgttt
      421 gcggactatc ccgcaactcg ccatactgtg gaccctgatc gccacatcga gagggtgact
      481 gagcttcagg agctgttcct gaccagggta gggctggaca taggcaaggt ctgggtggcc
      541 gacgatggag ccgctgttgc ggtgtggact accccagaga gcgtggaagc aggggctgtc
      601 tttgccgaaa ttgggccaag gatggccgaa ctgtctgggt cccgtttggc tgcccaacag
      661 cagatggaag gactgcttgc cccacatcga cccaaggagc ctgcatggtt cctcgctacc
      721 gtgggtgtga gcccagatca ccagggcaaa ggtctgggat cagccgtcgt tctgcctggc
      781 gtcgaggctg ctgagagag
//
