LOCUS       sequence-139702-         836 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  template base vector for N-terminal tagging, constitutive Puromycin 
            resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 836)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 836)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pRB35
FEATURES             Location/Qualifiers
     source          1..836
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    449..482
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 atgtcaaaag agacgtcttt tgttaagaat gctgaggaac ttgcaaagca aaaaatggat
       61 gctattaacc ctgaactttc ttcaaaattt aaatttttaa taaaattcct gtctcagttt
      121 cctgaagctt gctctaaacc tcgttcaaaa aaaatgcaga ataaagttgg tcaagaggaa
      181 catattgaat atttagctcg tagttttcat gagagtcgat tgccaagaaa acccacgcca
      241 cctacaacgg ttcctgatga ggtggttagc atagttctta atataagttt taatatacag
      301 cctgaaaatc ttgagagaat aaaagaagaa catcgatttt ccatggcagc tgagaatatt
      361 gtaggagatc ttctagaaag atactagtac cgccgcttgg agcagctgga gaggtaccga
      421 attcaccggt ggatcccatt ttggattaga agttcctatt ctctagaaag tataggaact
      481 tccctaggtt tgtgttaaaa tattttgttg ttgattatat taaaataaac ttgattcact
      541 tagccgttag ctagcactgt ttgatgattg tcgactggcg gtccagcatt cttatagagc
      601 tttcactgct ttgcgcacca aaaaaatcaa ttcctgttat cagggcgtgt gcaaatcgct
      661 tatcatcgcc gggcagaaaa agcattatgc ttttgcgcac gccgatcgac ttgcacacgg
      721 ctttcttatc ttatcgggca ccacggccca cagtttcgat tggactggat cgggtataaa
      781 gaggaggatt tcccaaccag aaggcatcaa caagttcaat aagcttctaa gactag
//
