LOCUS       sequence-139703-         840 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  template for N-terminal 3x FLAG tag, constitutive Puromycin 
            resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 840)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 840)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pRB33
FEATURES             Location/Qualifiers
     source          1..840
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(21..53)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 ctcgagtttt tcagcaagat aagttcctat actttctaga gaataggaac ttccatatgg
       61 gcgcgccctg ttggaatata ctattcaacc tacaaaaata acgttaaaca acactacttt
      121 atatttgata tgaatggcca caccttttat gccataaaac atattgtaag agaataccac
      181 tctttttatt ccttctttcc ttcttgtacg ttttttgctg tgagtaggtc gtggtgctgg
      241 tgttgcagtt gaaataactt aaaatataaa tcataaaact caaacataaa cttgactatt
      301 tatttattta ttaagaaagg aggtaccggt ggcgcgccat gaccgagtac aagcctacag
      361 tgcgactggc cacacgcgat gacgttccca gagccgttcg gacgttggct gccgcgtttg
      421 cggactatcc cgcaactcgc catactgtgg accctgatcg ccacatcgag agggtgactg
      481 agcttcagga gctgttcctg accagggtag ggctggacat aggcaaggtc tgggtggccg
      541 acgatggagc cgctgttgcg gtgtggacta ccccagagag cgtggaagca ggggctgtct
      601 ttgccgaaat tgggccaagg atggccgaac tgtctgggtc ccgtttggct gcccaacagc
      661 agatggaagg actgcttgcc ccacatcgac ccaaggagcc tgcatggttc ctcgctaccg
      721 tgggtgtgag cccagatcac cagggcaaag gtctgggatc agccgtcgtt ctgcctggcg
      781 tcgaggctgc tgagagagca ggagtaccgg cattcctcga aaccagtgct ccacggaacc
//
