LOCUS       sequence-139704-         868 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  template for N-terminal 3x FLAG tag, constitutive Puromycin 
            resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 868)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 868)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pRB33
FEATURES             Location/Qualifiers
     source          1..868
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    513..546
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 catgtcaaaa gagacgtctt ttgttaagaa tgctgaggaa cttgcaaagc aaaaaatgga
       61 tgctattaac cctgaacttt cttcaaaatt taaattttta ataaaattcc tgtctcagtt
      121 tcctgaagct tgctctaaac ctcgttcaaa aaaaatgcag aataaagttg gtcaagagga
      181 acatattgaa tatttagctc gtagttttca tgagagtcga ttgccaagaa aacccacgcc
      241 acctacaacg gttcctgatg aggtggttag catagttctt aatataagtt ttaatataca
      301 gcctgaaaat cttgagagaa taaaagaaga acatcgattt tccatggcag ctgagaatat
      361 tgtaggagat cttctagaaa gatactagta ccgccgcttg gagcagctgg agaggtaccc
      421 ttgtcgtcgt ccttgtagtc gatgtcgtgg tccttgtagt cgccgtcgtg gtccttgtag
      481 tcgaattcac cggtggatcc cattttggat tagaagttcc tattctctag aaagtatagg
      541 aacttcccta ggtttgtgtt aaaatatttt gttgttgatt atattaaaat aaacttgatt
      601 cacttagccg ttagctagca ctgtttgatg attgtcgact ggcggtccag cattcttata
      661 gagctttcac tgctttgcgc accaaaaaaa tcaattcctg ttatcagggc gtgtgcaaat
      721 cgcttatcat cgccgggcag aaaaagcatt atgcttttgc gcacgccgat cgacttgcac
      781 acggctttct tatcttatcg ggcaccacgg cccacagttt cgattggact ggatcgggta
      841 taaagaggag gatttcccaa ccagaagg
//
