LOCUS       sequence-139705-         843 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  template for N-terminal 1x Strep tag, constitutive Puromycin 
            resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 843)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 843)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pRB31
FEATURES             Location/Qualifiers
     source          1..843
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(13..45)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 tttcagcaag ataagttcct atactttcta gagaatagga acttccatat gggcgcgccc
       61 tgttggaata tactattcaa cctacaaaaa taacgttaaa caacactact ttatatttga
      121 tatgaatggc cacacctttt atgccataaa acatattgta agagaatacc actcttttta
      181 ttccttcttt ccttcttgta cgttttttgc tgtgagtagg tcgtggtgct ggtgttgcag
      241 ttgaaataac ttaaaatata aatcataaaa ctcaaacata aacttgacta tttatttatt
      301 tattaagaaa ggaggtaccg gtggcgcgcc atgaccgagt acaagcctac agtgcgactg
      361 gccacacgcg atgacgttcc cagagccgtt cggacgttgg ctgccgcgtt tgcggactat
      421 cccgcaactc gccatactgt ggaccctgat cgccacatcg agagggtgac tgagcttcag
      481 gagctgttcc tgaccagggt agggctggac ataggcaagg tctgggtggc cgacgatgga
      541 gccgctgttg cggtgtggac taccccagag agcgtggaag caggggctgt ctttgccgaa
      601 attgggccaa ggatggccga actgtctggg tcccgtttgg ctgcccaaca gcagatggaa
      661 ggactgcttg ccccacatcg acccaaggag cctgcatggt tcctcgctac cgtgggtgtg
      721 agcccagatc accagggcaa aggtctggga tcagccgtcg ttctgcctgg cgtcgaggct
      781 gctgagagag caggagtacc ggcattcctc gaaaccagtg ctccacggaa cctcccgttc
      841 tac
//
