LOCUS       sequence-139706-         800 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  template for N-terminal 1x Strep tag, constitutive Puromycin 
            resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 800)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 800)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pRB31
FEATURES             Location/Qualifiers
     source          1..800
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(429..452)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    477..510
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 gtcaaaagag acgtcttttg ttaagaatgc tgaggaactt gcaaagcaaa aaatggatgc
       61 tattaaccct gaactttctt caaaatttaa atttttaata aaattcctgt ctcagtttcc
      121 tgaagcttgc tctaaacctc gttcaaaaaa aatgcagaat aaagttggtc aagaggaaca
      181 tattgaatat ttagctcgta gttttcatga gagtcgattg ccaagaaaac ccacgccacc
      241 tacaacggtt cctgatgagg tggttagcat agttcttaat ataagtttta atatacagcc
      301 tgaaaatctt gagagaataa aagaagaaca tcgattttcc atggcagctg agaatattgt
      361 aggagatctt ctagaaagat actagtaccg ccgcttggag cagctggaga ggtaccgaat
      421 tcaccggttt tttcgaactg cgggtggctc caagcgctgg atcccatttt ggattagaag
      481 ttcctattct ctagaaagta taggaacttc cctaggtttg tgttaaaata ttttgttgtt
      541 gattatatta aaataaactt gattcactta gccgttagct agcactgttt gatgattgtc
      601 gactggcggt ccagcattct tatagagctt tcactgcttt gcgcaccaaa aaaatcaatt
      661 cctgttatca gggcgtgtgc aaatcgctta tcatcgccgg gcagaaaaag cattatgctt
      721 ttgcgcacgc cgatcgactt gcacacggct ttcttatctt atcgggcacc acggcccaca
      781 gtttcgattg gactggatcg
//
