LOCUS       sequence-139707-         831 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  template for N-terminal Strep-8xHis-Strep-TEV site tag, constitutive
            Puromycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 831)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 831)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pRB37
FEATURES             Location/Qualifiers
     source          1..831
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    complement(14..46)
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 ttttcagcaa gataagttcc tatactttct agagaatagg aacttccata tgggcgcgcc
       61 ctgttggaat atactattca acctacaaaa ataacgttaa acaacactac tttatatttg
      121 atatgaatgg ccacaccttt tatgccataa aacatattgt aagagaatac cactcttttt
      181 attccttctt tccttcttgt acgttttttg ctgtgagtag gtcgtggtgc tggtgttgca
      241 gttgaaataa cttaaaatat aaatcataaa actcaaacat aaacttgact atttatttat
      301 ttattaagaa aggaggtacc ggtggcgcgc catgaccgag tacaagccta cagtgcgact
      361 ggccacacgc gatgacgttc ccagagccgt tcggacgttg gctgccgcgt ttgcggacta
      421 tcccgcaact cgccatactg tggaccctga tcgccacatc gagagggtga ctgagcttca
      481 ggagctgttc ctgaccaggg tagggctgga cataggcaag gtctgggtgg ccgacgatgg
      541 agccgctgtt gcggtgtgga ctaccccaga gagcgtggaa gcaggggctg tctttgccga
      601 aattgggcca aggatggccg aactgtctgg gtcccgtttg gctgcccaac agcagatgga
      661 aggactgctt gccccacatc gacccaagga gcctgcatgg ttcctcgcta ccgtgggtgt
      721 gagcccagat caccagggca aaggtctggg atcagccgtc gttctgcctg gcgtcgaggc
      781 tgctgagaga gcaggagtac cggcattcct cgaaaccagt gctccacgga a
//
