LOCUS       sequence-139708-         776 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  template for N-terminal Strep-8xHis-Strep-TEV site tag, constitutive
            Puromycin resistance.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 776)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 776)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pRB37
FEATURES             Location/Qualifiers
     source          1..776
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(423..443)
                     /codon_start=1
                     /product="tobacco etch virus (TEV) protease recognition and
                     cleavage site"
                     /label=TEV site
                     /translation="ENLYFQG"
     CDS             complement(456..479)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     CDS             complement(486..509)
                     /codon_start=1
                     /product="8xHis affinity tag"
                     /label=8xHis
                     /translation="HHHHHHHH"
     CDS             complement(516..539)
                     /codon_start=1
                     /product="peptide that binds Strep-Tactin(R), an engineered
                     form of streptavidin"
                     /label=Strep-Tag II
                     /translation="WSHPQFEK"
     protein_bind    564..597
                     /label=FRT (minimal)
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="supports FLP-mediated excision but not integration 
                     (Turan and Bode, 2011)"
ORIGIN
        1 gtcaaaagag acgtcttttg ttaagaatgc tgaggaactt gcaaagcaaa aaatggatgc
       61 tattaaccct gaactttctt caaaatttaa atttttaata aaattcctgt ctcagtttcc
      121 tgaagcttgc tctaaacctc gttcaaaaaa aatgcagaat aaagttggtc aagaggaaca
      181 tattgaatat ttagctcgta gttttcatga gagtcgattg ccaagaaaac ccacgccacc
      241 tacaacggtt cctgatgagg tggttagcat agttcttaat ataagtttta atatacagcc
      301 tgaaaatctt gagagaataa aagaagaaca tcgattttcc atggcagctg agaatattgt
      361 aggagatctt ctagaaagat actagtaccg ccgcttggag cagctggaga ggtaccgaat
      421 tcgccctgaa aatacaggtt ttcgaattca ccggtttttt cgaactgcgg gtggctccac
      481 gatccgtgat gatggtgatg gtggtgatgt ccacctttct cgaactgcgg gtggctccaa
      541 gcgctggatc ccattttgga ttagaagttc ctattctcta gaaagtatag gaacttccct
      601 aggtttgtgt taaaatattt tgttgttgat tatattaaaa taaacttgat tcacttagcc
      661 gttagctagc actgtttgat gattgtcgac tggcggtcca gcattcttat agagctttca
      721 ctgctttgcg caccaaaaaa atcaattcct gttatcaggg cgtgtgcaaa tcgctt
//
