LOCUS       sequence-139712-         730 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  expression of improved FLP recombinase.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 730)
  AUTHORS   Kunzelmann S, Bottcher R, Schmidts I, Forstemann K
  TITLE     A Comprehensive Toolbox for Genome Editing in Cultured Drosophila 
            melanogaster Cells.
  JOURNAL   G3 (Bethesda). 2016 Jun 1;6(6):1777-85. doi: 10.1534/g3.116.028241.
  PUBMED    27172193
REFERENCE   2  (bases 1 to 730)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1534/g3.116"; 
            journalName: "G3 (Bethesda)"; date: "2016-06-1- 1"; volume: "6"; 
            issue: "6"; pages: "1777-85"
COMMENT     Sequence Label: pKF295
FEATURES             Location/Qualifiers
     source          1..730
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     170..188
                     /label=pCasper-F
                     /note="Drosophila mini-white gene, reverse primer"
     primer_bind     complement(210..229)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     polyA_signal    239..320
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     264..283
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
ORIGIN
        1 acagcgctga ctttgagtgg aatgtcattt tgagtgagag gtaatcgaaa gaacctggta
       61 catcaaatac ccttggatcg aagtaaattt aaaactgatc agataagttc aatgatatcc
      121 agtgcagtaa aaaaaaaaaa tgtttttttt atctactttc cgcaaaaatg ggttttatta
      181 acttacatac atactagaat tcatacattg atgagtttgg acaaaccaca actagaatgc
      241 agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta
      301 taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg
      361 gggaggtgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt atggctgatt
      421 atgatctaga gtcgcggccg cttatatgcg tctatttatg taggatgaaa ggtagtctag
      481 tacctcctgt gatattatcc cattccatgc ggggtatcgt atgcttcctt cagcactacc
      541 ctttagctgt tctatatgct gccactcctc aattggatta gtctcatcct tcaatgctat
      601 catttccttt gataatggat catatgcata gtaccgagaa actagtgcga agtagtgatc
      661 aggtattgct gttatctgat gagtatacgt tgtcctggcc acggcagaag cacgcttatc
      721 gctccaattt
//
