LOCUS       sequence-139720-        1089 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses myc-dL5(E52D)-TM (PDGFR derived) on the surface of 
            mammalian cells, with an Igk-leader. (MBIC5, dL5**, FAP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1089)
  AUTHORS   Telmer CA, Verma R, Teng H, Andreko S, Law L, Bruchez MP
  TITLE     Rapid, specific, no-wash, far-red fluorogen activation in 
            subcellular compartments by targeted fluorogen activating proteins.
  JOURNAL   ACS Chem Biol. 2015 May 15;10(5):1239-46. doi: 10.1021/cb500957k. 
            Epub 2015 Feb 16.
  PUBMED    25650487
REFERENCE   2  (bases 1 to 1089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/cb500957k";
            journalName: "ACS Chem Biol"; date: "2015-05-15- 15"; volume: "10"; 
            issue: "5"; pages: "1239-46"
COMMENT     Sequence Label: pcDNA3.1-kappa-myc-dL5-2xG4S-TMst
FEATURES             Location/Qualifiers
     source          1..1089
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(34..180)
                     /codon_start=1
                     /product="transmembrane domain from platelet derived growth
                     factor receptor beta"
                     /label=PDGFR-beta TM domain
                     /translation="AVGQDTQEVIVVPHSLPFKVVVISAILALVVLTIISLIILIMLWQ
                     KKPR"
     CDS             complement(250..969)
                     /codon_start=1
                     /product="fluorogen-activating protein composed of a dimer 
                     of the L5-MG single-chain antibody fragment (Szent-Gyorgyi 
                     et al., 2008)"
                     /label=FAP-alpha-2
                     /note="binds malachite green derivatives to produce red 
                     fluorescence"
                     /translation="QAVVTQEPSVTVSPGGTVILTCGSGTGAVTSGHYANWFQQKPGQA
                     PRALIFDTDKKYSWTPGRFSGSLLGAKAALTISDAQPEDEAEYYCSLSDVDGYLFGGGT
                     QLTVLSGGGGSGGGGSGGGGSGGGGSQAVVTQEPSVTVSPGGTVILTCGSGTGAVTSGH
                     YANWFQQKPGQAPRALIFDTDKKYSWTPGRFSGSLLGAKAALTISDAQPEDEAEYYCSL
                     SDVDGYLFGGGTQLTVLS"
     CDS             complement(985..1014)
                     /codon_start=1
                     /product="Myc (human c-Myc proto-oncogene) epitope tag"
                     /label=Myc
                     /translation="EQKLISEEDL"
     sig_peptide     complement(1024..1083)
                     /codon_start=1
                     /product="leader sequence from mouse immunoglobulin kappa 
                     light chain"
                     /label=Ig-kappa leader
                     /translation="METDTLLLWVLLLWVPGSTG"
ORIGIN
        1 gggccctcta gagtcgacag ctgcctcgac ttaacgtggc ttcttctgcc aaagcatgat
       61 gaggatgata agggagatga tggtgagcac caccagggcc aggatggctg agatcaccac
      121 caccttaaag ggcaaggagt gtggcaccac gatgacctcc tgcgtgtcct ggcccacagc
      181 ctcgagtaag ataccggtgc tgccaccgcc accagaccca ccgcctccca tatgggccag
      241 accggccgcg gagaggacgg tcagctgggt gcctcctccg aacagataac cgtcaacgtc
      301 ggagagcgaa cagtaatact cagcctcatc ttcaggctgc gcatccgaga tggtcagggc
      361 agccttggcc ccaaggaggg agcctgagaa tcggccaggg gtccaggaat acttcttgtc
      421 ggtgtcaaat ataagtgccc tgggggcttg gccaggcttc tgctggaacc agttggcata
      481 atgaccactg gtgacagctc cagtgccgga gccacaagtg agaatgactg tccctcctgg
      541 ggacacagtc actgacggct cctgagtcac cacagcctga gaaccgccgc ctccggaacc
      601 accaccgccg ctgccaccgc caccagagcc gccaccaccg gacagaaccg tcagttgtgt
      661 tccaccgcca aacaagtagc catccacgtc gctaagagaa cagtaatact cggcttcgtc
      721 ctcaggctga gcatctgata ttgtcaaagc tgcctttgct cccagcaaag atccagaaaa
      781 tctacctggg gtccaagaat acttcttatc agtatcaaaa atcaaagctc ttggagcttg
      841 tcctggtttt tgttgaaacc aatttgcata atgaccagat gtgacggcac ccgtaccact
      901 tccacaagta agtataacag taccacctgg gctaacggta acactaggtt cttgggtaac
      961 gacggcctgc aggccagaaa ggcccaggtc ctcctcgctg atcagcttct gctcgaattc
     1021 gtcaccagtg gaacctggaa cccagagcag cagtacccat agcaggagtg tgtctgtctc
     1081 catggtgga
//
