LOCUS       sequence-139722-        1016 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dL5(E52D)-mCer3 fusion protein in cytosol of mammalian 
            cells, penetrates nucleus. (MBIC5, dL5**, FAP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1016)
  AUTHORS   Telmer CA, Verma R, Teng H, Andreko S, Law L, Bruchez MP
  TITLE     Rapid, specific, no-wash, far-red fluorogen activation in 
            subcellular compartments by targeted fluorogen activating proteins.
  JOURNAL   ACS Chem Biol. 2015 May 15;10(5):1239-46. doi: 10.1021/cb500957k. 
            Epub 2015 Feb 16.
  PUBMED    25650487
REFERENCE   2  (bases 1 to 1016)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/cb500957k";
            journalName: "ACS Chem Biol"; date: "2015-05-15- 15"; volume: "10"; 
            issue: "5"; pages: "1239-46"
COMMENT     Sequence Label: pcDNA3.1-KozATG-dL5-2XG4S-mCer3
FEATURES             Location/Qualifiers
     source          1..1016
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             169..888
                     /codon_start=1
                     /product="fluorogen-activating protein composed of a dimer 
                     of the L5-MG single-chain antibody fragment (Szent-Gyorgyi 
                     et al., 2008)"
                     /label=FAP-alpha-2
                     /note="binds malachite green derivatives to produce red 
                     fluorescence"
                     /translation="QAVVTQEPSVTVSPGGTVILTCGSGTGAVTSGHYANWFQQKPGQA
                     PRALIFDTDKKYSWTPGRFSGSLLGAKAALTISDAQPEDEAEYYCSLSDVDGYLFGGGT
                     QLTVLSGGGGSGGGGSGGGGSGGGGSQAVVTQEPSVTVSPGGTVILTCGSGTGAVTSGH
                     YANWFQQKPGQAPRALIFDTDKKYSWTPGRFSGSLLGAKAALTISDAQPEDEAEYYCSL
                     SDVDGYLFGGGTQLTVLS"
     primer_bind     complement(991..1012)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcgt ttaaacttaa gcttgccacc
      121 ggatccggct tggggatatc caccatggaa ttcggccttt ctggcctgca ggccgtcgtt
      181 acccaagaac ctagtgttac cgttagccca ggtggtactg ttatacttac ttgtggaagt
      241 ggtacgggtg ccgtcacatc tggtcattat gcaaattggt ttcaacaaaa accaggacaa
      301 gctccaagag ctttgatttt tgatactgat aagaagtatt cttggacccc aggtagattt
      361 tctggatctt tgctgggagc aaaggcagct ttgacaatat cagatgctca gcctgaggac
      421 gaagccgagt attactgttc tcttagcgac gtggatggct acttgtttgg cggtggaaca
      481 caactgacgg ttctgtccgg tggtggcggc tctggtggcg gtggcagcgg cggtggtggt
      541 tccggaggcg gcggttctca ggctgtggtg actcaggagc cgtcagtgac tgtgtcccca
      601 ggagggacag tcattctcac ttgtggctcc ggcactggag ctgtcaccag tggtcattat
      661 gccaactggt tccagcagaa gcctggccaa gcccccaggg cacttatatt tgacaccgac
      721 aagaagtatt cctggacccc tggccgattc tcaggctccc tccttggggc caaggctgcc
      781 ctgaccatct cggatgcgca gcctgaagat gaggctgagt attactgttc gctctccgac
      841 gttgacggtt atctgttcgg aggaggcacc cagctgaccg tcctctccgc ggccggtctg
      901 gcccatatgg gaggcggtgg gtctggtggc ggtggcagca ccggtatggt gagcaagggc
      961 gaggagctgt tcaccggggt ggtgcccatc ctggtcgagc tggacggcga cgtaaa
//
