LOCUS       sequence-139723-        1003 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dL5(E52D)-mCer3 fusion protein in cytosol of mammalian 
            cells, penetrates nucleus. (MBIC5, dL5**, FAP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1003)
  AUTHORS   Telmer CA, Verma R, Teng H, Andreko S, Law L, Bruchez MP
  TITLE     Rapid, specific, no-wash, far-red fluorogen activation in 
            subcellular compartments by targeted fluorogen activating proteins.
  JOURNAL   ACS Chem Biol. 2015 May 15;10(5):1239-46. doi: 10.1021/cb500957k. 
            Epub 2015 Feb 16.
  PUBMED    25650487
REFERENCE   2  (bases 1 to 1003)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/cb500957k";
            journalName: "ACS Chem Biol"; date: "2015-05-15- 15"; volume: "10"; 
            issue: "5"; pages: "1239-46"
COMMENT     Sequence Label: pcDNA3.1-KozATG-dL5-2XG4S-mCer3
FEATURES             Location/Qualifiers
     source          1..1003
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(31..744,745..747,748..750))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(76..97)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     425..444
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     684..705
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 gggccctcta gagtcgacag ctgcctcgag ttacttgtac agctcgtcca tgccgagagt
       61 gatcccggcg gcggtcacga actccagcag gaccatgtga tcgcgcttct cgttggggtc
      121 tttgctcagc ttggactggg tgctcaggta gtggttgtcg ggcagcagca cggggccgtc
      181 gccgatgggg gtgttctgct ggtagtggtc ggcgagctgc acgctgccgt cctcgatgtt
      241 gcagttgagg ccgaagttgg ccttgatgcc gttcttctgc ttgtcggcgg tgatatagac
      301 gttgccgtgg atggcgttgt actccagctt gtgccccagg atgttgccgt cctccttgaa
      361 gtcgatgccc ttcagctcga tgcggttcac cagggtgtcg ccctcgaact tcacctcggc
      421 gcgggtcttg tagttgccgt cgtccttgaa gaagatggtg cgctcctgga cgtagccttc
      481 gggcatggcg gacttgaaga agtcgtgctg cttcatgtgg tcggggtagc gggcgaagca
      541 ctgcacgccc cagctcaggg tggtcacgag ggtgggccag ggcacgggca gcttgccggt
      601 ggtgcagatg aacttcaggg tcagcttgcc gtaggtggca tcgccctcgc cctcgccgga
      661 cacgctgaac ttgtggccgt ttacgtcgcc gtccagctcg accaggatgg gcaccacccc
      721 ggtgaacagc tcctcgccct tgctcaccat accggtgctg ccaccgccac cagacccacc
      781 gcctcccata tgggccagac cggccgcgga gaggacggtc agctgggtgc ctcctccgaa
      841 cagataaccg tcaacgtcgg agagcgaaca gtaatactca gcctcatctt caggctgcgc
      901 atccgagatg gtcagggcag ccttggcccc aaggagggag cctgagaatc ggccaggggt
      961 ccaggaatac ttcttgtcgg tgtcaaatat aagtgccctg ggg
//
