LOCUS       sequence-139726-         984 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dL5(E52D)-mCer3 fusion protein in mitochondria of 
            mammalian cells. (MBIC5, dL5**, FAP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Telmer CA, Verma R, Teng H, Andreko S, Law L, Bruchez MP
  TITLE     Rapid, specific, no-wash, far-red fluorogen activation in 
            subcellular compartments by targeted fluorogen activating proteins.
  JOURNAL   ACS Chem Biol. 2015 May 15;10(5):1239-46. doi: 10.1021/cb500957k. 
            Epub 2015 Feb 16.
  PUBMED    25650487
REFERENCE   2  (bases 1 to 984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/cb500957k";
            journalName: "ACS Chem Biol"; date: "2015-05-15- 15"; volume: "10"; 
            issue: "5"; pages: "1239-46"
COMMENT     Sequence Label: pcDNA3.1-COXIV-COX8-dL5-2XG4S-mCer3
FEATURES             Location/Qualifiers
     source          1..984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     61..80
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        61..79
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     CDS             220..294
                     /codon_start=1
                     /product="mitochondrial presequence of human cytochrome c 
                     oxidase subunit VIII (Rizutto et al., 1989)"
                     /label=COX8 presequence
                     /translation="MSVLTPLLLRGLTGSARRLPVPRAK"
ORIGIN
        1 ctatataagc agagctctct ggctaactag agaacccact gcttactggc ttatcgaaat
       61 taatacgact cactataggg agacccaagc tggctagcgt ttaaacttaa gcttgccacc
      121 ggatccgcca ccatgctcgc tacaagagtt ttctcacttg tcggcaaaag agccatttct
      181 acctcggttt gtgtaagggc tgaagaatct agagaattca tgtccgtcct gacgccgctg
      241 ctgctgcggg gcttgacagg ctcggcccgg cggctcccag tgccgcgcgc caagatccat
      301 tcgttgccgc cggaggggaa gcttgaattc ggcctttctg gcctgcaggc cgtcgttacc
      361 caagaaccta gtgttaccgt tagcccaggt ggtactgtta tacttacttg tggaagtggt
      421 acgggtgccg tcacatctgg tcattatgca aattggtttc aacaaaaacc aggacaagct
      481 ccaagagctt tgatttttga tactgataag aagtattctt ggaccccagg tagattttct
      541 ggatctttgc tgggagcaaa ggcagctttg acaatatcag atgctcagcc tgaggacgaa
      601 gccgagtatt actgttctct tagcgacgtg gatggctact tgtttggcgg tggaacacaa
      661 ctgacggttc tgtccggtgg tggcggctct ggtggcggtg gcagcggcgg tggtggttcc
      721 ggaggcggcg gttctcaggc tgtggtgact caggagccgt cagtgactgt gtccccagga
      781 gggacagtca ttctcacttg tggctccggc actggagctg tcaccagtgg tcattatgcc
      841 aactggttcc agcagaagcc tggccaagcc cccagggcac ttatatttga caccgacaag
      901 aagtattcct ggacccctgg ccgattctca ggctccctcc ttggggccaa ggctgccctg
      961 accatctcgg atgcgcagcc tgaa
//
