LOCUS       sequence-139727-         933 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses dL5(E52D)-mCer3 fusion protein in mitochondria of 
            mammalian cells. (MBIC5, dL5**, FAP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 933)
  AUTHORS   Telmer CA, Verma R, Teng H, Andreko S, Law L, Bruchez MP
  TITLE     Rapid, specific, no-wash, far-red fluorogen activation in 
            subcellular compartments by targeted fluorogen activating proteins.
  JOURNAL   ACS Chem Biol. 2015 May 15;10(5):1239-46. doi: 10.1021/cb500957k. 
            Epub 2015 Feb 16.
  PUBMED    25650487
REFERENCE   2  (bases 1 to 933)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/cb500957k";
            journalName: "ACS Chem Biol"; date: "2015-05-15- 15"; volume: "10"; 
            issue: "5"; pages: "1239-46"
COMMENT     Sequence Label: pcDNA3.1-COXIV-COX8-dL5-2XG4S-mCer3
FEATURES             Location/Qualifiers
     source          1..933
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(34..747,748..750,751..753))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(79..100)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     428..447
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     687..708
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 aacgggccct ctagagtcga cagctgcctc gagttacttg tacagctcgt ccatgccgag
       61 agtgatcccg gcggcggtca cgaactccag caggaccatg tgatcgcgct tctcgttggg
      121 gtctttgctc agcttggact gggtgctcag gtagtggttg tcgggcagca gcacggggcc
      181 gtcgccgatg ggggtgttct gctggtagtg gtcggcgagc tgcacgctgc cgtcctcgat
      241 gttgcagttg aggccgaagt tggccttgat gccgttcttc tgcttgtcgg cggtgatata
      301 gacgttgccg tggatggcgt tgtactccag cttgtgcccc aggatgttgc cgtcctcctt
      361 gaagtcgatg cccttcagct cgatgcggtt caccagggtg tcgccctcga acttcacctc
      421 ggcgcgggtc ttgtagttgc cgtcgtcctt gaagaagatg gtgcgctcct ggacgtagcc
      481 ttcgggcatg gcggacttga agaagtcgtg ctgcttcatg tggtcggggt agcgggcgaa
      541 gcactgcacg ccccagctca gggtggtcac gagggtgggc cagggcacgg gcagcttgcc
      601 ggtggtgcag atgaacttca gggtcagctt gccgtaggtg gcatcgccct cgccctcgcc
      661 ggacacgctg aacttgtggc cgtttacgtc gccgtccagc tcgaccagga tgggcaccac
      721 cccggtgaac agctcctcgc ccttgctcac cataccggtg ctgccaccgc caccagaccc
      781 accgcctccc atatgggcca gaccggccgc ggagaggacg gtcagctggg tgcctcctcc
      841 gaacagataa ccgtcaacgt cggagagcga acagtaatac tcagcctcat cttcaggctg
      901 cgcatccgag atggtcaggg cagccttggc ccc
//
