LOCUS       sequence-139732-        6367 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  JxOn-pre.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6367)
  AUTHORS   Druckmann S, Feng L, Lee B, Yook C, Zhao T, Magee JC, Kim J
  TITLE     Structured Synaptic Connectivity between Hippocampal Regions.
  JOURNAL   Neuron. 2014 Feb 5;81(3):629-40. doi: 10.1016/j.neuron.2013.11.026. 
            Epub 2014 Jan 9.
  PUBMED    24412418
REFERENCE   2  (bases 1 to 6367)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1016/j.neuron.2013.11.026"; journalName: "Neuron"; date: 
            "2014-02-5- 5"; volume: "81"; issue: "3"; pages: "629-40"
COMMENT     Sequence Label: paavCAG-JxON-pre-mGRASP-mCerulean
FEATURES             Location/Qualifiers
     source          1..6367
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        1..278
                     /label=chicken beta-actin promoter
     primer_bind     557..579
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     641..660
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     protein_bind    complement(692..725)
                     /label=lox66
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     CDS             complement(2325..2372)
                     /codon_start=1
                     /product="11th beta-strand of superfolder GFP"
                     /label=GFP11
                     /note="associates with GFP(1-10), consisting of the first 
                     10 beta-strands, to reconstitute fluorescent GFP (Cabantous
                     et al., 2005; Kamiyama et al., 2016)"
                     /translation="RDHMVLHEYVNAAGIT"
     protein_bind    2472..2505
                     /label=lox71
                     /bound_moiety="Cre recombinase"
                     /note="Left element (LE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (ATGTATGC) (Shaw et al., 2021)."
     misc_feature    2518..3106
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(2571..2591)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2989..3000)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     polyA_signal    3149..3270
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(3160..3179)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     3214..3233
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     rep_origin      complement(3649..4237)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(3729..3748)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(4408..5199,5200..5268))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     5031..5050
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(5269..5373)
                     /gene="bla"
                     /label=AmpR promoter
     rep_origin      complement(5399..5854)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(5536..5557)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     5748..5767
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     repeat_region   5926..6055
                     /label=AAV2 ITR
ORIGIN
        1 tcgaggtgag ccccacgttc tgcttcactc tccccatctc ccccccctcc ccacccccaa
       61 ttttgtattt atttattttt taattatttt gtgcagcgat gggggcgggg gggggggggg
      121 ggcgcgcgcc aggcggggcg gggcggggcg aggggcgggg cggggcgagg cggagaggtg
      181 cggcggcagc caatcagagc ggcgcgctcc gaaagtttcc ttttatggcg aggcggcggc
      241 ggcggcggcc ctataaaaag cgaagcgcgc ggcgggcggg agtcgctgcg cgctgccttc
      301 gccccgtgcc ccgctccgcc gccgcctcgc gccgcccgcc ccggctctga ctgaccgcgt
      361 tactcccaca ggtgagcggg cgggacggcc cttctcctcc gggctgtaat tagcgcttgg
      421 tttaatgacg gcttgtttct tttctgtggc tgcgtgaaag ccttgagggg ctccgggagg
      481 gccctttgtg cggggggagc ggctcggggc tgtccgcggg gggacggctg ccttcggggg
      541 ggacggggca gggcggggtt cggcttctgg cgtgtgaccg gcggctctag agcctctgct
      601 aaccatgttc atgccttctt ctttttccta cagctcctgg gcaacgtgct ggttattgtg
      661 ctgtctcatc attttggcaa agaattggat cataacttcg tatagcatac attatacgaa
      721 cggtaaagct tgatgtcaaa ctctcacttg tacagctcat ccatacccag ggttatgccg
      781 gcagctgtga caaactccag cagtaccata tgatctctct tttcgttggg gtccttgctc
      841 agcttgctct gtgtactaag gtagtgattg tcgggcaaca gcacaggtcc atcaccgatg
      901 ggagtattct gttggtaatg gtcagccagc tgaacgcttc catcctctat gttgtgccgg
      961 attttgaaat tggcctttat gccatttttc tgcttgtcgg cggttatgta aacattatca
     1021 gatatggcgt tgtattccag cttgtggccg agaatgttcc catcttcttt gaagtcgatt
     1081 cccttgagtt caatcctgtt caccaaggtg tcaccttcga atttcacctc cgcccttgtc
     1141 ttgtagttac catcgtcctt gaaaaatatc gtcctttcct gaacataccc ctcgggcata
     1201 gcggacttga agaagtcgtg ctgcttcatg tggtcagggt accgggcgaa gcactgtaca
     1261 ccccatgtga gggtggtgac gagtgtgggc caaggcacgg ggagcttccc tgttgtacag
     1321 atgaacttca gcgtcagttt gccgtaggtg gcatcccctt ctccctcgcc gcttacggag
     1381 aacttgtgcc catttacatc tccgtcgagc tccaccagga taggcacgac ccctgtaaac
     1441 aactcttcgc ccttgctcac catagatctt acgtagtact ctttatcctt gtttttcttg
     1501 tttttgtttg ctgatttggc gctggagggc tgtttctctt taaccacggc gccattactc
     1561 tgagcgctgt tactgatata gttcctagat tcgtcaacgt ggtaagatcc ctcatccctg
     1621 ttgcgatact tgtagcgtac gcagaagaag atgcccaggc cgataaacag aagcaggccg
     1681 gcgactcccc cgagtacgat cagggccatc ggctgtactg gtgttgacca agtaggaagc
     1741 accttgatgt tgctctccag cagcacctgt ccagagtcgc taagaagaca ctgccacatc
     1801 cctgcctccg ggttaaggac ccacacagcc ttctctcttt tactgacttt agcctcctta
     1861 ttctccagct tcagagacag catcagttta ggtgaggtgg gaccccacac ttcgcaggtc
     1921 aggttcttct gcagctgtgt tgccctcatc accacgaggt tgacctcctg atgcaacttt
     1981 ccggtcttag cctccaaggc gagggtgagg ttcccggagc cagcatactg gggcagggct
     2041 tgggggagtg tcaagtgcaa aggcaacttc ttgcccatct gcagcttagg gtcctgtgtg
     2101 actctcttca cgctcacttc tttgtttttc agatcaaaag tgatccaact cttgctggag
     2161 gaggctcttt cggcctgcca ccacaactct cctgatcctg tgagcttctc gactgtgaag
     2221 gccaatggga aactgaactc cacctgttcc ccctccttct tataaacaat agagctagct
     2281 ttctgaaagt cgacgctacc accgccgcca gatccgccac cgcctgtgat gccagcagcg
     2341 ttcacatatt cgtgcagcac catgtggtcg cgggaagcgg tgacttctcc tgtcttccaa
     2401 tctgaagcgg gaagagcgaa ggggagaagt gcagcaagca acaaaagact agtagaaggt
     2461 ggcatggatc tataacttcg tataatgtat gctatacgaa cggtacagct tatcgataat
     2521 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
     2581 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
     2641 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
     2701 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
     2761 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
     2821 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
     2881 ggcactgaca attccgtggt gttgtcgggg aaatcatcgt cctttccttg gctgctcgcc
     2941 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
     3001 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
     3061 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcatcg ataccgtcga
     3121 cccgggcggc cgcttcgagc agacatgata agatacattg atgagtttgg acaaaccaca
     3181 actagaatgc agtgaaaaaa atgctttatt tgtgaaattt gtgatgctat tgctttattt
     3241 gtaaccatta taagctgcaa taaacaagtt aacaacaaca attgcattca ttttatgttt
     3301 caggttcagg gggagatgtg ggaggttttt taaagcaagt aaaacctcta caaatgtggt
     3361 aaaatcgata aggatcttcc tagagcatgg ctacgtagat aagtagcatg gcgggttaat
     3421 cattaactac aaggaacccc tagtgatgga gttggccact ccctctctgc gcgctcgctc
     3481 gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa
     3541 ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa
     3601 aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct
     3661 ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac
     3721 aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc
     3781 gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc
     3841 tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg
     3901 tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga
     3961 gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta acaggattag
     4021 cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta
     4081 cactagaaga acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag
     4141 agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg
     4201 caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac
     4261 ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc
     4321 aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag
     4381 tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc
     4441 agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac
     4501 gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc
     4561 accggctcca gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg
     4621 tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag
     4681 tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc
     4741 acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac
     4801 atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag
     4861 aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac
     4921 tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg
     4981 agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc
     5041 gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact
     5101 ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg
     5161 atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa
     5221 tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt
     5281 tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg
     5341 tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctaa
     5401 attgtaagcg ttaatatttt gttaaaattc gcgttaaatt tttgttaaat cagctcattt
     5461 tttaaccaat aggccgaaat cggcaaaatc ccttataaat caaaagaata gaccgagata
     5521 gggttgagtg ttgttccagt ttggaacaag agtccactat taaagaacgt ggactccaac
     5581 gtcaaagggc gaaaaaccgt ctatcagggc gatggcccac tacgtgaacc atcaccctaa
     5641 tcaagttttt tggggtcgag gtgccgtaaa gcactaaatc ggaaccctaa agggagcccc
     5701 cgatttagag cttgacgggg aaagccggcg aacgtggcga gaaaggaagg gaagaaagcg
     5761 aaaggagcgg gcgctagggc gctggcaagt gtagcggtca cgctgcgcgt aaccaccaca
     5821 cccgccgcgc ttaatgcgcc gctacagggc gcgtcccatt cgccattcag gctgcgcaac
     5881 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctgcg cgctcgctcg
     5941 ctcactgagg ccgcccgggc aaagcccggg cgtcgggcga cctttggtcg cccggcctca
     6001 gtgagcgagc gagcgcgcag agagggagtg gccaactcca tcactagggg ttccttgtag
     6061 ttaatgatta acccgccatg ctacttatct acgtagccat gctctaggaa gatcgtacca
     6121 ttgacgtcaa taatgacgta tgttcccata gtaacgccaa tagggacttt ccattgacgt
     6181 caatgggtgg agtatttacg gtaaactgcc cacttggcag tacatcaagt gtatcatatg
     6241 ccaagtacgc cccctattga cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag
     6301 tacatgacct tatgggactt tcctacttgg cagtacatct acgtattagt catcgctatt
     6361 accatgg
//
