LOCUS       sequence-139733-        1050 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses myc-dL5(E52D)-mCer3 fusion protein in endoplasmic 
            reticulum of mammalian cells with an Igk-leader. (MBIC5, dL5**, 
            FAP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1050)
  AUTHORS   Telmer CA, Verma R, Teng H, Andreko S, Law L, Bruchez MP
  TITLE     Rapid, specific, no-wash, far-red fluorogen activation in 
            subcellular compartments by targeted fluorogen activating proteins.
  JOURNAL   ACS Chem Biol. 2015 May 15;10(5):1239-46. doi: 10.1021/cb500957k. 
            Epub 2015 Feb 16.
  PUBMED    25650487
REFERENCE   2  (bases 1 to 1050)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/cb500957k";
            journalName: "ACS Chem Biol"; date: "2015-05-15- 15"; volume: "10"; 
            issue: "5"; pages: "1239-46"
COMMENT     Sequence Label: pcDNA3.1-kappa-myc-dL5-2XG4S-mCer3-KDEL
FEATURES             Location/Qualifiers
     source          1..1050
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(82..792,793..795,796..798))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(124..145)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     473..492
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     732..753
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 aacgggccct ctagagtcga cagctgcctc gacctcgaga aagacgagtt gtgataatca
       61 ttacagttca tccttctcga gcttgtacag ctcgtccatg ccgagagtga tcccggcggc
      121 ggtcacgaac tccagcagga ccatgtgatc gcgcttctcg ttggggtctt tgctcagctt
      181 ggactgggtg ctcaggtagt ggttgtcggg cagcagcacg gggccgtcgc cgatgggggt
      241 gttctgctgg tagtggtcgg cgagctgcac gctgccgtcc tcgatgttgc agttgaggcc
      301 gaagttggcc ttgatgccgt tcttctgctt gtcggcggtg atatagacgt tgccgtggat
      361 ggcgttgtac tccagcttgt gccccaggat gttgccgtcc tccttgaagt cgatgccctt
      421 cagctcgatg cggttcacca gggtgtcgcc ctcgaacttc acctcggcgc gggtcttgta
      481 gttgccgtcg tccttgaaga agatggtgcg ctcctggacg tagccttcgg gcatggcgga
      541 cttgaagaag tcgtgctgct tcatgtggtc ggggtagcgg gcgaagcact gcacgcccca
      601 gctcagggtg gtcacgaggg tgggccaggg cacgggcagc ttgccggtgg tgcagatgaa
      661 cttcagggtc agcttgccgt aggtggcatc gccctcgccc tcgccggaca cgctgaactt
      721 gtggccgttt acgtcgccgt ccagctcgac caggatgggc accaccccgg tgaacagctc
      781 ctcgcccttg ctcaccatac cggtgctgcc accgccacca gacccaccgc ctcccatatg
      841 ggccagaccg gccgcggaga ggacggtcag ctgggtgcct cctccgaaca gataaccgtc
      901 aacgtcggag agcgaacagt aatactcagc ctcatcttca ggctgcgcat ccgagatggt
      961 cagggcagcc ttggccccaa ggagggagcc tgagaatcgg ccaggggtcc aggaatactt
     1021 cttgtcggtg tcaaatataa gtgccctggg
//
