LOCUS       sequence-139736-         865 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 865)
  AUTHORS   Cirera-Salinas D, Yu J, Bodak M, Ngondo RP, Herbert KM, Ciaudo C
  TITLE     Noncanonical function of DGCR8 controls mESC exit from pluripotency.
  JOURNAL   J Cell Biol. 2017 Jan 18. pii: jcb.201606073. doi: 
            10.1083/jcb.201606073.
  PUBMED    28100686
REFERENCE   2  (bases 1 to 865)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2017 Jan 18. pii: jcb.201606073. doi: 10.1083/jcb.201606073."
COMMENT     Sequence Label: pX330-sgRNA_Dgcr8_3
FEATURES             Location/Qualifiers
     source          1..865
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(1..48)
                     /codon_start=1
                     /product="bipartite nuclear localization signal from 
                     nucleoplasmin"
                     /label=nucleoplasmin NLS
                     /translation="KRPAATKKAGQAKKKK"
ORIGIN
        1 ctttttcttt tttgcctggc cggccttttt cgtggccgcc ggccttttgt cgcctcccag
       61 ctgagacagg tcgatccgtg tctcgtacag gccggtgatg ctctggtgga tcagggtggc
      121 gtccagcacc tctttggtgc tggtgtacct cttccggtcg atggtggtgt caaagtactt
      181 gaaggcggca ggggctccca gattggtcag ggtaaacagg tggatgatat tctcggcctg
      241 ctctctgatg ggcttatccc ggtgcttgtt gtaggcggac agcactttgt ccagattagc
      301 gtcggccagg atcactctct tggagaactc gctgatctgc tcgatgatct cgtccaggta
      361 gtgcttgtgc tgttccacaa acagctgttt ctgctcatta tcctcggggg agcccttcag
      421 cttctcatag tggctggcca ggtacaggaa gttcacatat ttggagggca gggccagttc
      481 gtttcccttc tgcagttcgc cggcagaggc cagcattctc ttccggccgt tttccagctc
      541 gaacagggag tacttaggca gcttgatgat caggtccttt ttcacttctt tgtagccctt
      601 ggcttccaga aagtcgatgg gattcttctc gaagctgctt ctttccatga tggtgatccc
      661 cagcagctct ttcacactct tcagtttctt ggacttgccc ttttccactt tggccaccac
      721 cagcacagaa taggccacgg tggggctgtc gaagccgccg tacttcttag ggtcccagtc
      781 cttctttctg gcgatcagct tatcgctgtt cctcttgggc aggatagact ctttgctgaa
      841 gccgcctgtc tgcacctcgg tcttt
//
