LOCUS       sequence-139738-        9716 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9716)
  AUTHORS   Buganim Y, Itskovich E, Hu YC, Cheng AW, Ganz K, Sarkar S, Fu D, 
            Welstead GG, Page DC, Jaenisch R
  TITLE     Direct Reprogramming of Fibroblasts into Embryonic Sertoli-like 
            Cells by Defined Factors.
  JOURNAL   Cell Stem Cell. 2012 Sep 7;11(3):373-86.
  PUBMED    22958931
REFERENCE   2  (bases 1 to 9716)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell."; date: "2012-09-7"; volume: "11(3)"; pages: "373-86"
COMMENT     Sequence Label: FUW-TetO-Wt1
FEATURES             Location/Qualifiers
     source          1..9716
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     protein_bind    41..59
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    83..101
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    125..143
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    167..185
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    209..227
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     promoter        260..298
                     /label=minimal CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     295..319
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     CDS             1219..1230
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     misc_feature    1835..2423
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     primer_bind     complement(1888..1908)
                     /label=WPRE-R
                     /note="WPRE, reverse primer"
     CDS             complement(2306..2317)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     primer_bind     complement(2426..2442)
                     /label=KS primer
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(2427..2443)
                     /label=pBluescriptKS
                     /note="For pBluescript vector"
     LTR             2948..3128
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     primer_bind     complement(3154..3171)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    3160..3384
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     rep_origin      3430..3858
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(3517..3536)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     3727..3748
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(3867..3887)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        3872..4201
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4052..4187
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     4114..4133
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     promoter        4249..4296
                     /label=EM7 promoter
                     /note="synthetic bacterial promoter "
     CDS             4315..4689
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /label=BleoR
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
                     VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
                     EFALRDPAGNCVHFVAEEQD"
     polyA_signal    4819..4940
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(4856..4875)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     4910..4929
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(4989..5005)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(4989..5005)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(5002..5024)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    5013..5029
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(5037..5043,5044..5061,5062..5067))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5082..5103
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(5220..5237)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(5391..5979)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(5471..5490)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(6150..6941,6942..7010))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     6773..6792
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(7011..7115)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     complement(7190..7209)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     enhancer        7381..7760
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        7762..7960
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     LTR             7978..8158
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    8205..8330
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    8823..9056
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             9241..9285
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9583..9700
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
ORIGIN
        1 acagcagaga tccagtttgg ttagatctcg agtttaccac tccctatcag tgatagagaa
       61 aagtgaaagt cgagtttacc actccctatc agtgatagag aaaagtgaaa gtcgagttta
      121 ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc tatcagtgat
      181 agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa gtgaaagtcg
      241 agctcggtac ccgggtcgag gtaggcgtgt acggtgggag gcctatataa gcagagctcg
      301 tttagtgaac cgtcagatcg cctggagacg ccatccacgc tgttttgacc tccatagaag
      361 acaccgggac cgatccagcc tccgcggccc cgaattcgcc cttctggact tcctcctgtc
      421 gcaggagccg gcttccacgt gtgtcccgga gccggcgtct cagcacacgc tccgccggga
      481 gcccgggtgc gtccagcagc cggagcaacc tggggaccga ggcccccgga gcgcctgggc
      541 caagtccagc gccgagaatc cgcaggatcg caggagcgga gaaccgtccg catccgagcc
      601 gcacctcatg ggttccgacg tgcgggacct gaacgcgctg ctgcccgctg tgcctccgcc
      661 gccaccccac tccttcatca aacaggagcc cagctggggc ggcgccgagc cacacgagga
      721 gcagtgcctg agcgccttca ccttgcactt ctcgggccag ttcaccggta cagccggggc
      781 ctgtcgctac ggacccttcg gtcctccccc gcccagccag gcgtcctcgg gccaggccag
      841 gatgttcccc aatgcgccct acctgcccag ctgcctggag agccagccta ccatccgcaa
      901 ccaaggatac agcacggtca ctttcgacgg ggcgcccagc tatggccaca cgccctcgca
      961 tcacgcggcg cagttcccca accattcctt caaacacgag gaccccatgg gccagcaggg
     1021 ctcgctgggc gagcagcagt actccgtgcc acctccggtg tatggctgcc acacccctac
     1081 tgacagttgc acaggcagcc aggccctgct cctgaggacg ccctacagca gtgacaattt
     1141 ataccaaatg acctcccagc ttgaatgcat gacctggaat cagatgaacc taggagctac
     1201 cttaaaggga atggctgctg ggagctccag ctcagtgaaa tggacagaag ggcagagcaa
     1261 ccacggcaca gggtacgaga gtgagaacca cacggccccc atcctctgtg gtgcccagta
     1321 cagaatacac acccacgggg tcttccgagg cattcaggat gtgcggcgtg tatctggagt
     1381 ggccccaact cttgtccggt cagcatctga aaccagtgag aaacgtcctt tcatgtgtgc
     1441 atacccaggc tgcaataaga gatattttaa gctgtcccac ttacagatgc atagccggaa
     1501 gcacactggt gagaaaccat accagtgtga cttcaaggac tgcgagagaa ggttttctcg
     1561 ctcagaccag ctcaaaagac accaaaggag acacacaggt gtgaaaccat tccagtgtaa
     1621 aacttgtcag cgaaagtttt cccggtccga ccatctgaag acccacacca ggactcatac
     1681 aggtgaaaag cccttcagct gtcggtggca cagttgtcag aaaaagtttg cgcgctcaga
     1741 cgaattggtc cgccatcaca acatgcatca gagaaacatg accaaactcc agctggcgct
     1801 ttgaaagggc gaattcgata tcaagcttat cgataatcaa cctctggatt acaaaatttg
     1861 tgaaagattg actggtattc ttaactatgt tgctcctttt acgctatgtg gatacgctgc
     1921 tttaatgcct ttgtatcatg ctattgcttc ccgtatggct ttcattttct cctccttgta
     1981 taaatcctgg ttgctgtctc tttatgagga gttgtggccc gttgtcaggc aacgtggcgt
     2041 ggtgtgcact gtgtttgctg acgcaacccc cactggttgg ggcattgcca ccacctgtca
     2101 gctcctttcc gggactttcg ctttccccct ccctattgcc acggcggaac tcatcgccgc
     2161 ctgccttgcc cgctgctgga caggggctcg gctgttgggc actgacaatt ccgtggtgtt
     2221 gtcggggaaa tcatcgtcct ttccttggct gctcgcctgt gttgccacct ggattctgcg
     2281 cgggacgtcc ttctgctacg tcccttcggc cctcaatcca gcggaccttc cttcccgcgg
     2341 cctgctgccg gctctgcggc ctcttccgcg tcttcgcctt cgccctcaga cgagtcggat
     2401 ctccctttgg gccgcctccc cgcatcgata ccgtcgacct cgagacctag aaaaacatgg
     2461 agcaatcaca agtagcaata cagcagctac caatgctgat tgtgcctggc tagaagcaca
     2521 agaggaggag gaggtgggtt ttccagtcac acctcaggta cctttaagac caatgactta
     2581 caaggcagct gtagatctta gccacttttt aaaagaaaag gggggactgg aagggctaat
     2641 tcactcccaa cgaagacaag atatccttga tctgtggatc taccacacac aaggctactt
     2701 ccctgattgg cagaactaca caccagggcc agggatcaga tatccactga cctttggatg
     2761 gtgctacaag ctagtaccag ttgagcaaga gaaggtagaa gaagccaatg aaggagagaa
     2821 cacccgcttg ttacaccctg tgagcctgca tgggatggat gacccggaga gagaagtatt
     2881 agagtggagg tttgacagcc gcctagcatt tcatcacatg gcccgagagc tgcatccgga
     2941 ctgtactggg tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg
     3001 gaacccactg cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg
     3061 tctgttgtgt gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat
     3121 ctctagcagg gcccgtttaa acccgctgat cagcctcgac tgtgccttct agttgccagc
     3181 catctgttgt ttgcccctcc cccgtgcctt ccttgaccct ggaaggtgcc actcccactg
     3241 tcctttccta ataaaatgag gaaattgcat cgcattgtct gagtaggtgt cattctattc
     3301 tggggggtgg ggtggggcag gacagcaagg gggaggattg ggaagacaat agcaggcatg
     3361 ctggggatgc ggtgggctct atggcttctg aggcggaaag aaccagctgg ggctctaggg
     3421 ggtatcccca cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca
     3481 gcgtgaccgc tacacttgcc agcgccctag cgcccgctcc tttcgctttc ttcccttcct
     3541 ttctcgccac gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt
     3601 tccgatttag tgctttacgg cacctcgacc ccaaaaaact tgattagggt gatggttcac
     3661 gtagtgggcc atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct
     3721 ttaatagtgg actcttgttc caaactggaa caacactcaa ccctatctcg gtctattctt
     3781 ttgatttata agggattttg ccgatttcgg cctattggtt aaaaaatgag ctgatttaac
     3841 aaaaatttaa cgcgaattaa ttctgtggaa tgtgtgtcag ttagggtgtg gaaagtcccc
     3901 aggctcccca gcaggcagaa gtatgcaaag catgcatctc aattagtcag caaccaggtg
     3961 tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     4021 agcaaccata gtcccgcccc taactccgcc catcccgccc ctaactccgc ccagttccgc
     4081 ccattctccg ccccatggct gactaatttt ttttatttat gcagaggccg aggccgcctc
     4141 tgcctctgag ctattccaga agtagtgagg aggctttttt ggaggcctag gcttttgcaa
     4201 aaagctcccg ggagcttgta tatccatttt cggatctgat cagcacgtgt tgacaattaa
     4261 tcatcggcat agtatatcgg catagtataa tacgacaagg tgaggaacta aaccatggcc
     4321 aagttgacca gtgccgttcc ggtgctcacc gcgcgcgacg tcgccggagc ggtcgagttc
     4381 tggaccgacc ggctcgggtt ctcccgggac ttcgtggagg acgacttcgc cggtgtggtc
     4441 cgggacgacg tgaccctgtt catcagcgcg gtccaggacc aggtggtgcc ggacaacacc
     4501 ctggcctggg tgtgggtgcg cggcctggac gagctgtacg ccgagtggtc ggaggtcgtg
     4561 tccacgaact tccgggacgc ctccgggccg gccatgaccg agatcggcga gcagccgtgg
     4621 gggcgggagt tcgccctgcg cgacccggcc ggcaactgcg tgcacttcgt ggccgaggag
     4681 caggactgac acgtgctacg agatttcgat tccaccgccg ccttctatga aaggttgggc
     4741 ttcggaatcg ttttccggga cgccggctgg atgatcctcc agcgcgggga tctcatgctg
     4801 gagttcttcg cccaccccaa cttgtttatt gcagcttata atggttacaa ataaagcaat
     4861 agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg tggtttgtcc
     4921 aaactcatca atgtatctta tcatgtctgt ataccgtcga cctctagcta gagcttggcg
     4981 taatcatggt catagctgtt tcctgtgtga aattgttatc cgctcacaat tccacacaac
     5041 atacgagccg gaagcataaa gtgtaaagcc tggggtgcct aatgagtgag ctaactcaca
     5101 ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa acctgtcgtg ccagctgcat
     5161 taatgaatcg gccaacgcgc ggggagaggc ggtttgcgta ttgggcgctc ttccgcttcc
     5221 tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc gagcggtatc agctcactca
     5281 aaggcggtaa tacggttatc cacagaatca ggggataacg caggaaagaa catgtgagca
     5341 aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg
     5401 ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg
     5461 acaggactat aaagatacca ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt
     5521 ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc cttcgggaag cgtggcgctt
     5581 tctcatagct cacgctgtag gtatctcagt tcggtgtagg tcgttcgctc caagctgggc
     5641 tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt
     5701 gagtccaacc cggtaagaca cgacttatcg ccactggcag cagccactgg taacaggatt
     5761 agcagagcga ggtatgtagg cggtgctaca gagttcttga agtggtggcc taactacggc
     5821 tacactagaa gaacagtatt tggtatctgc gctctgctga agccagttac cttcggaaaa
     5881 agagttggta gctcttgatc cggcaaacaa accaccgctg gtagcggtgg tttttttgtt
     5941 tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt gatcttttct
     6001 acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt catgagatta
     6061 tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa atcaatctaa
     6121 agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga ggcacctatc
     6181 tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt gtagataact
     6241 acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg agacccacgc
     6301 tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga gcgcagaagt
     6361 ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga agctagagta
     6421 agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg catcgtggtg
     6481 tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc aaggcgagtt
     6541 acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc
     6601 agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca taattctctt
     6661 actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac caagtcattc
     6721 tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg ggataatacc
     6781 gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa
     6841 ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg tgcacccaac
     6901 tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa
     6961 aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat actcttcctt
     7021 tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata catatttgaa
     7081 tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa agtgccacct
     7141 gacgtcgacg gatcgggaga tctcccgatc ccctatggtg cactctcagt acaatctgct
     7201 ctgatgccgc atagttaagc cagtatctgc tccctgcttg tgtgttggag gtcgctgagt
     7261 agtgcgcgag caaaatttaa gctacaacaa ggcaaggctt gaccgacaat tgcatgaaga
     7321 atctgcttag ggttaggcgt tttgcgctgc ttcgcgatgt acgggccaga tatacgcgtt
     7381 gacattgatt attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc
     7441 catatatgga gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca
     7501 acgacccccg cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga
     7561 ctttccattg acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc
     7621 aagtgtatca tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct
     7681 ggcattatgc ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat
     7741 tagtcatcgc tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc
     7801 ggtttgactc acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt
     7861 ggcaccaaaa tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa
     7921 tgggcggtag gcgtgtacgg tgggaggtct atataagcag cgcgttttgc ctgtactggg
     7981 tctctctggt tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg
     8041 cttaagcctc aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt
     8101 gactctggta actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt
     8161 ggcgcccgaa cagggacttg aaagcgaaag ggaaaccaga ggagctctct cgacgcagga
     8221 ctcggcttgc tgaagcgcgc acggcaagag gcgaggggcg gcgactggtg agtacgccaa
     8281 aaattttgac tagcggaggc tagaaggaga gagatgggtg cgagagcgtc agtattaagc
     8341 gggggagaat tagatcgcga tgggaaaaaa ttcggttaag gccaggggga aagaaaaaat
     8401 ataaattaaa acatatagta tgggcaagca gggagctaga acgattcgca gttaatcctg
     8461 gcctgttaga aacatcagaa ggctgtagac aaatactggg acagctacaa ccatcccttc
     8521 agacaggatc agaagaactt agatcattat ataatacagt agcaaccctc tattgtgtgc
     8581 atcaaaggat agagataaaa gacaccaagg aagctttaga caagatagag gaagagcaaa
     8641 acaaaagtaa gaccaccgca cagcaagcgg ccgctgatct tcagacctgg aggaggagat
     8701 atgagggaca attggagaag tgaattatat aaatataaag tagtaaaaat tgaaccatta
     8761 ggagtagcac ccaccaaggc aaagagaaga gtggtgcaga gagaaaaaag agcagtggga
     8821 ataggagctt tgttccttgg gttcttggga gcagcaggaa gcactatggg cgcagcgtca
     8881 atgacgctga cggtacaggc cagacaatta ttgtctggta tagtgcagca gcagaacaat
     8941 ttgctgaggg ctattgaggc gcaacagcat ctgttgcaac tcacagtctg gggcatcaag
     9001 cagctccagg caagaatcct ggctgtggaa agatacctaa aggatcaaca gctcctgggg
     9061 atttggggtt gctctggaaa actcatttgc accactgctg tgccttggaa tgctagttgg
     9121 agtaataaat ctctggaaca gatttggaat cacacgacct ggatggagtg ggacagagaa
     9181 attaacaatt acacaagctt aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa
     9241 aagaatgaac aagaattatt ggaattagat aaatgggcaa gtttgtggaa ttggtttaac
     9301 ataacaaatt ggctgtggta tataaaatta ttcataatga tagtaggagg cttggtaggt
     9361 ttaagaatag tttttgctgt actttctata gtgaatagag ttaggcaggg atattcacca
     9421 ttatcgtttc agacccacct cccaaccccg aggggacccg acaggcccga aggaatagaa
     9481 gaagaaggtg gagagagaga cagagacaga tccattcgat tagtgaacgg atcggcactg
     9541 cgtgcgccaa ttctgcagac aaatggcagt attcatccac aattttaaaa gaaaaggggg
     9601 gattgggggg tacagtgcag gggaaagaat agtagacata atagcaacag acatacaaac
     9661 taaagaatta caaaaacaaa ttacaaaaat tcaaaatttt cgggtttatt acaggg
//
