LOCUS       sequence-139739-         707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Cirera-Salinas D, Yu J, Bodak M, Ngondo RP, Herbert KM, Ciaudo C
  TITLE     Noncanonical function of DGCR8 controls mESC exit from pluripotency.
  JOURNAL   J Cell Biol. 2017 Jan 18. pii: jcb.201606073. doi: 
            10.1083/jcb.201606073.
  PUBMED    28100686
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2017 Jan 18. pii: jcb.201606073. doi: 10.1083/jcb.201606073."
COMMENT     Sequence Label: pX330-sgRNA_Dgcr8_4
FEATURES             Location/Qualifiers
     source          1..707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     109..128
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        207..282
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        379..664
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 attggaatta atttgactgt aaacacaaag atattagtac aaaatacgtg acgtagaaag
       61 taataatttc ttgggtagtt tgcagtttta aaattatgtt ttaaaatgga ctatcatatg
      121 cttaccgtaa cttgaaagta tttcgatttc ttggctttat atatcttgtg gaaaggacga
      181 aacaccggca gcactcggcc tgagtggttt tagagctaga aatagcaagt taaaataagg
      241 ctagtccgtt atcaacttga aaaagtggca ccgagtcggt gcttttttgt tttagagcta
      301 gaaatagcaa gttaaaataa ggctagtccg tttttagcgc gtgcgccaat tctgcagaca
      361 aatggctcta gaggtacccg ttacataact tacggtaaat ggcccgcctg gctgaccgcc
      421 caacgacccc cgcccattga cgtcaatagt aacgccaata gggactttcc attgacgtca
      481 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
      541 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt gtgcccagta
      601 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
      661 catggtcgag gtgagcccca cgttctgctt cactctcccc atctccc
//
