LOCUS       sequence-139745-        1082 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses myc-dL5(E52D)-mCer3 fusion protein in peroxisomes of 
            mammalian cells with a C-terminal PTS (SKL). (MBIC5, dL5**, FAP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1082)
  AUTHORS   Telmer CA, Verma R, Teng H, Andreko S, Law L, Bruchez MP
  TITLE     Rapid, specific, no-wash, far-red fluorogen activation in 
            subcellular compartments by targeted fluorogen activating proteins.
  JOURNAL   ACS Chem Biol. 2015 May 15;10(5):1239-46. doi: 10.1021/cb500957k. 
            Epub 2015 Feb 16.
  PUBMED    25650487
REFERENCE   2  (bases 1 to 1082)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/cb500957k";
            journalName: "ACS Chem Biol"; date: "2015-05-15- 15"; volume: "10"; 
            issue: "5"; pages: "1239-46"
COMMENT     Sequence Label: pcDNA3.1-KozATG-myc-dL5-2XG4S-mCer3-SKL
FEATURES             Location/Qualifiers
     source          1..1082
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             complement(join(45..755,756..758,759..761))
                     /codon_start=1
                     /product="enhanced monomeric variant of CFP (Rizzo et al., 
                     2004)"
                     /label=mCerulean
                     /note=" mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLSWGVQCFARYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNAIHGNVYITADKQKNGIK
                     ANFGLNCNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(87..108)
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     436..455
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     695..716
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
ORIGIN
        1 acgggccctc tagagtcgac agctgcctcg agttacagct tgctcttgta cagctcgtcc
       61 atgccgagag tgatcccggc ggcggtcacg aactccagca ggaccatgtg atcgcgcttc
      121 tcgttggggt ctttgctcag cttggactgg gtgctcaggt agtggttgtc gggcagcagc
      181 acggggccgt cgccgatggg ggtgttctgc tggtagtggt cggcgagctg cacgctgccg
      241 tcctcgatgt tgcagttgag gccgaagttg gccttgatgc cgttcttctg cttgtcggcg
      301 gtgatataga cgttgccgtg gatggcgttg tactccagct tgtgccccag gatgttgccg
      361 tcctccttga agtcgatgcc cttcagctcg atgcggttca ccagggtgtc gccctcgaac
      421 ttcacctcgg cgcgggtctt gtagttgccg tcgtccttga agaagatggt gcgctcctgg
      481 acgtagcctt cgggcatggc ggacttgaag aagtcgtgct gcttcatgtg gtcggggtag
      541 cgggcgaagc actgcacgcc ccagctcagg gtggtcacga gggtgggcca gggcacgggc
      601 agcttgccgg tggtgcagat gaacttcagg gtcagcttgc cgtaggtggc atcgccctcg
      661 ccctcgccgg acacgctgaa cttgtggccg tttacgtcgc cgtccagctc gaccaggatg
      721 ggcaccaccc cggtgaacag ctcctcgccc ttgctcacca taccggtgct gccaccgcca
      781 ccagacccac cgcctcccat atgggccaga ccggccgcgg agaggacggt cagctgggtg
      841 cctcctccga acagataacc gtcaacgtcg gagagcgaac agtaatactc agcctcatct
      901 tcaggctgcg catccgagat ggtcagggca gccttggccc caaggaggga gcctgagaat
      961 cggccagggg tccaggaata cttcttgtcg gtgtcaaata taagtgccct gggggcttgg
     1021 ccaggcttct gctggaacca gttggcataa tgaccactgg tgacagctcc agtgccggag
     1081 cc
//
