LOCUS       sequence-139750-        1025 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression vector for dL5(E52D) protein with N-terminal 
            His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1025)
  AUTHORS   Wang Y, Telmer CA, Schmidt BF, Franke JD, Ort S, Arndt-Jovin DJ, 
            Bruchez MP
  TITLE     Fluorogen activating protein-affibody probes: modular, no-wash 
            measurement of epidermal growth factor receptors.
  JOURNAL   Bioconjug Chem. 2015 Jan 21;26(1):137-44. doi: 10.1021/bc500525b. 
            Epub 2014 Dec 19.
  PUBMED    25490520
REFERENCE   2  (bases 1 to 1025)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/bc500525b";
            journalName: "Bioconjug Chem"; date: "2015-01-21- 21"; volume: "26";
            issue: "1"; pages: "137-44"
COMMENT     Sequence Label: pET21-10XHis-GST-HRV-dL5
FEATURES             Location/Qualifiers
     source          1..1025
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     65..84
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        65..83
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     protein_bind    84..108
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     RBS             123..145
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     CDS             158..187
                     /codon_start=1
                     /product="10xHis affinity tag"
                     /label=10xHis
                     /translation="HHHHHHHHHH"
     CDS             191..844
                     /codon_start=1
                     /product="glutathione S-transferase from Schistosoma 
                     japonicum"
                     /label=GST
                     /translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
                     FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
                     GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
                     YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
     primer_bind     802..824
                     /label=pGEX 5'
                     /note="pGEX vectors, Glutathione-S-transferase, forward 
                     primer"
     CDS             866..889
                     /codon_start=1
                     /product="recognition and cleavage site for human 
                     rhinovirus 3C and PreScission proteases"
                     /label=HRV 3C site
                     /translation="LEVLFQGP"
ORIGIN
        1 ggcgccggtg atgccggcca cgatgcgtcc ggcgtagagg atcgagatct cgatcccgcg
       61 aaattaatac gactcactat aggggaattg tgagcggata acaattcccc tctagaaata
      121 attttgttta actttaagaa ggagatatac atatatgcac catcatcacc accatcatca
      181 ccaccatagc atgtccccta tactaggtta ttggaaaatt aagggccttg tgcaacccac
      241 tcgacttctt ttggaatatc ttgaagaaaa atatgaagag catttgtatg agcgcgatga
      301 aggtgataaa tggcgaaaca aaaagtttga attgggtttg gagtttccca atcttcctta
      361 ttatattgat ggtgatgtta aattaacaca gtctatggcc atcatacgtt atatagctga
      421 caagcacaac atgttgggtg gttgtccaaa agagcgtgca gagatttcaa tgcttgaagg
      481 agcggttttg gatattagat acggtgtttc gagaattgca tatagtaaag actttgaaac
      541 tctcaaagtt gattttctta gcaagctacc tgaaatgctg aaaatgttcg aagatcgttt
      601 atgtcataaa acatatttaa atggtgatca tgtaacccat cctgacttca tgttgtatga
      661 cgctcttgat gttgttttat acatggaccc aatgtgcctg gatgcgttcc caaaattagt
      721 ttgttttaaa aaacgtattg aagctatccc acaaattgat aagtacttga aatccagcaa
      781 gtatatagca tggcctttgc agggctggca agccacgttt ggtggtggtg accatcctcc
      841 aaaatcggat tctccgaatt gcggcctgga agttctgttc cagggtccgg gatcccttga
      901 attccaggcc gtcgttaccc aagaacctag tgttaccgtt agcccaggtg gtactgttat
      961 acttacttgt ggaagtggta cgggtgccgt cacatctggt cattatgcaa attggtttca
     1021 acaaa
//
