LOCUS       sequence-139752-         757 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Bacterial expression vector for dL5(E52D) protein with N-terminal 
            His10, GST and HRV3C cleavage site (MBIC5, dL5**, FAP).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 757)
  AUTHORS   Wang Y, Telmer CA, Schmidt BF, Franke JD, Ort S, Arndt-Jovin DJ, 
            Bruchez MP
  TITLE     Fluorogen activating protein-affibody probes: modular, no-wash 
            measurement of epidermal growth factor receptors.
  JOURNAL   Bioconjug Chem. 2015 Jan 21;26(1):137-44. doi: 10.1021/bc500525b. 
            Epub 2014 Dec 19.
  PUBMED    25490520
REFERENCE   2  (bases 1 to 757)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1021/bc500525b";
            journalName: "Bioconjug Chem"; date: "2015-01-21- 21"; volume: "26";
            issue: "1"; pages: "137-44"
COMMENT     Sequence Label: pET21-10XHis-GST-HRV-dL5
FEATURES             Location/Qualifiers
     source          1..757
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     32..50
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     CDS             complement(103..120)
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
ORIGIN
        1 caagacccgt ttagaggccc caaggggtta tgctagttat tgctcagcgg tggcagcagc
       61 caactcagct tcctttcggg ctttgttagc agccggatct cagtggtggt ggtggtggtg
      121 ctcgactcat tcattcactc gagtaagata ccggtggaga ggacggtcag ctgggtgcct
      181 cctccgaaca gataaccgtc aacgtcggag agcgaacagt aatactcagc ctcatcttca
      241 ggctgcgcat ccgagatggt cagggcagcc ttggccccaa ggagggagcc tgagaatcgg
      301 ccaggggtcc aggaatactt cttgtcggtg tcaaatataa gtgccctggg ggcttggcca
      361 ggcttctgct ggaaccagtt ggcataatga ccactggtga cagctccagt gccggagcca
      421 caagtgagaa tgactgtccc tcctggggac acagtcactg acggctcctg agtcaccaca
      481 gcctgagaac cgccgcctcc ggaaccacca ccgccgctgc caccgccacc agagccgcca
      541 ccaccggaca gaaccgtcag ttgtgttcca ccgccaaaca agtagccatc cacgtcgcta
      601 agagaacagt aatactcggc ttcgtcctca ggctgagcat ctgatattgt caaagctgcc
      661 tttgctccca gcaaagatcc agaaaatcta cctggggtcc aagaatactt cttatcagta
      721 tcaaaaatca aagctcttgg agcttgtcct ggttttt
//
