LOCUS       sequence-139755-        2157 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Mammalian expression of C-terminal fusion of mRuby3 to CX43.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 2157)
  AUTHORS   Bajar BT, Wang ES, Lam AJ, Kim BB, Jacobs CL, Howe ES, Davidson MW, 
            Lin MZ, Chu J
  TITLE     Improving brightness and photostability of green and red fluorescent
            proteins for live cell imaging and FRET reporting.
  JOURNAL   Sci Rep. 2016 Feb 16;6:20889. doi: 10.1038/srep20889.
  PUBMED    26879144
REFERENCE   2  (bases 1 to 2157)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Sci Rep."; 
            date: "2016-02-16"; volume: "6:20889. doi"; pages: " 
            10.1038/srep20889"
COMMENT     Sequence Label: pKanCMV-CX4.3-7aa-mRuby3
FEATURES             Location/Qualifiers
     source          1..2157
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      1399..1408
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             1405..2115
                     /codon_start=1
                     /product="monomeric red fluorescent protein derived from 
                     mRuby2, with improved brightness and photostability (Bajar 
                     et al., 2016)"
                     /label=mRuby3
                     /translation="MVSKGEELIKENMRMKVVMEGSVNGHQFKCTGEGEGRPYEGVQTM
                     RIKVIEGGPLPFAFDILATSFMYGSRTFIKYPADIPDFFKQSFPEGFTWERVTRYEDGG
                     VVTVTQDTSLEDGELVYNVKVRGVNFPSNGPVMQKKTKGWEPNTEMMYPADGGLRGYTD
                     IALKVDGGGHLHCNFVTTYRSKKTVGNIKMPGVHAVDHRLERIEESDNETYVVQREVAV
                     AKYSNLGGGMDELYK"
ORIGIN
        1 atccgctagc gctaccggac tcagatctcg agctcaagct tcgaattcgg cagcactttt
       61 ctttcattgg ggggaaggcg tgaggaaagt accaaacagc agcagacttt taaactttaa
      121 acagacaggt ctgagagcct gaactctcat ttttcctttg acttcagcct ccaaggagtt
      181 ccaccaactt tggcgcgccg gcttcacttt cattaagtga aagagaggtg cccagacatg
      241 ggtgactgga gtgccttggg gaagcttctg gacaaggtcc aagcctactc caccgctgga
      301 gggaaggtgt ggctgtcagt gctcttcata ttcagaatcc tgctcctggg gacagctgtt
      361 gagtcagctt ggggtgatga acagtctgcc tttcgctgta acactcaaca acctggctgc
      421 gaaaacgtct gctatgacaa gtccttcccc atctctcacg tgcgcttctg ggtccttcag
      481 atcatattcg tgtctgtgcc cacactcctg tacttggccc atgtgttcta tgtgatgagg
      541 aaggaagaga agctaaacaa gaaagaagag gagctcaaag tggcccagac tgacggggtc
      601 aacgtggaga tgcacctgaa gcagattgaa atcaagaagt tcaagtacgg gattgaagag
      661 cacggcaagg tgaaaatgag gggcggcttg ctgagaacct acatcatcag catcctcttc
      721 aagtctgtct tcgaggtggc cttcctgctc atccagtggt acatctatgg gttcagcttg
      781 agcgcggtct acacctgcaa gagagatccc tgcccccacc aggtagactg cttcctctca
      841 cgtcccacgg agaaaaccat cttcatcatc ttcatgctgg tggtgtcctt ggtgtctctc
      901 gctttgaaca tcattgagct cttctacgtc ttcttcaaag gcgttaagga tcgcgtgaag
      961 ggaagaagcg atccttacca cgccaccact ggcccactga gcccatcaaa agactgcgga
     1021 tctccaaaat acgcctactt caatggctgc tcctcaccaa cggctccact ctcgcctatg
     1081 tctcctcctg ggtacaagct ggttactggt gacagaaaca attcctcgtg ccgcaattac
     1141 aacaagcaag ctagcgagca aaactgggcg aactacagcg cagagcaaaa tcgcatgggg
     1201 caggccggaa gcaccatctc caactcgcac gcccagccgt tcgatttccc cgacgacaac
     1261 cagaatgcca aaaaagttgc tgctggacat gaactccagc cattagccat cgtggaccaa
     1321 cgaccttcca gcagagccag cagccgcgcc agcagcaggc ctcggcctga tgacctggag
     1381 attgcggatc caccggtcgc caccatggtg tctaagggcg aagagctgat caaggaaaat
     1441 atgcgtatga aggtggtcat ggaaggttcg gtcaacggcc accaattcaa atgcacaggt
     1501 gaaggagaag gcagaccgta cgagggagtg caaaccatga ggatcaaagt catcgaggga
     1561 ggacccctgc catttgcctt tgacattctt gccacgtcgt tcatgtatgg cagccgtacc
     1621 tttatcaagt acccggccga catccctgat ttctttaaac agtcctttcc tgagggtttt
     1681 acttgggaaa gagttacgag atacgaagat ggtggagtcg tcaccgtcac gcaggacacc
     1741 agccttgagg atggcgagct cgtctacaac gtcaaggtca gaggggtaaa ctttccctcc
     1801 aatggtcccg tgatgcagaa gaagaccaag ggttgggagc ctaatacaga gatgatgtat
     1861 ccagcagatg gtggtctgag aggatacact gacatcgcac tgaaagttga tggtggtggc
     1921 catctgcact gcaacttcgt gacaacttac aggtcaaaaa agaccgtcgg gaacatcaag
     1981 atgcccggtg tccatgccgt tgatcaccgc ctggaaagga tcgaggagag tgacaatgaa
     2041 acctacgtag tgcaaagaga agtggcagtt gccaaataca gcaaccttgg tggtggcatg
     2101 gacgagctgt acaagtaagc ggccgcgact ctagatcata atcagccata ccacatt
//
