LOCUS       sequence-139766-         917 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 917)
  AUTHORS   Ichida M, Finkel T
  TITLE     Ras regulates NFAT3 activity in cardiac myocytes.
  JOURNAL   J Biol Chem. 2001 Feb 2. 276(5):3524-30.
  PUBMED    11044444
REFERENCE   2  (bases 1 to 917)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Biol 
            Chem. 2001 Feb 2. 276(5):3524-30."
COMMENT     Sequence Label: NFAT luciferase reporter
FEATURES             Location/Qualifiers
     source          1..917
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     91..109
                     /label=LucNrev
                     /note="Firefly luciferase, reverse primer"
     protein_bind    291..320
                     /label=NFAT binding site
                     /bound_moiety="NFAT transcription factor"
                     /note="NFAT binding site from the human interleukin 2 
                     (IL-2) gene (Mattila et al., 1990)"
     protein_bind    321..350
                     /label=NFAT binding site
                     /bound_moiety="NFAT transcription factor"
                     /note="NFAT binding site from the human interleukin 2 
                     (IL-2) gene (Mattila et al., 1990)"
     protein_bind    351..380
                     /label=NFAT binding site
                     /bound_moiety="NFAT transcription factor"
                     /note="NFAT binding site from the human interleukin 2 
                     (IL-2) gene (Mattila et al., 1990)"
     polyA_signal    419..540
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(430..449)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     484..503
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(867..888)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
ORIGIN
        1 tttcgaagta ttccgcgtac gtgatgttca cctcgatatg tgcatctgta aaagcaattg
       61 ttccaggaac cagggcgtat ctcttcatag ccttatgcag ttgctctcca gcggttccat
      121 cctctagagg atagaatggc gccgggcctt tctttatgtt tttggcgtct tccattttac
      181 caacagtacc ggaatgccaa gcttgcgggt cgctgcaggg tcgctcggtg ttcgaggcca
      241 cacgcgtcac cttaatatgc gaagtggacc tgggaccgcg ccagatcccc ggaggaaaaa
      301 ctgtttcata cagaaggcgt ggaggaaaaa ctgtttcata cagaaggcgt ggaggaaaaa
      361 ctgtttcata cagaaggcgt ggtacctccc gggttatgtt agctcagtta cagtaccata
      421 agatacattg atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt
      481 tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt
      541 aacaacaaca attgcattca ttttatgttt caggttcagg gggaggtgtg ggaggttttt
      601 taaagcaagt aaaacctcca cgtaccttaa tattacttac ttatcatggt agcttgggct
      661 ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg
      721 gcgaatggca aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa
      781 tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat
      841 agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg
      901 tggactccaa cgtcaaa
//
