LOCUS       sequence-139776-         647 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SpCas9, GFP, and sgRNA targeting Ago1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 647)
  AUTHORS   Ngondo RP, Cirera-Salinas D, Yu J, Wischnewski H, Bodak M, 
            Vandormael-Pournin S, Geiselmann A, Wettstein R, Luitz J, 
            Cohen-Tannoudji M, Ciaudo C
  TITLE     Argonaute 2 Is Required for Extra-embryonic Endoderm Differentiation
            of Mouse Embryonic Stem Cells.
  JOURNAL   Stem Cell Reports. 2018 Jan 24. pii: S2213-6711(17)30571-4. doi: 
            10.1016/j.stemcr.2017.12.023.
  PUBMED    29396181
REFERENCE   2  (bases 1 to 647)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2018 Jan 24. pii: S2213-6711(17)30571-4. doi: 
            10.1016/j.stemcr.2017.12.023."
COMMENT     Sequence Label: pX458-sgRNA_Ago1_4
FEATURES             Location/Qualifiers
     source          1..647
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             11..31
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
ORIGIN
        1 taagatggcc ccaaagaaga agcggaaggt cgggtatcca cggagtccca gcagccgaca
       61 agaagtacag catcggcctg gacatcggca ccaactctgt gggctgggcc gtgatcaccg
      121 acgagtacaa ggtgcccagc aagaaattca aggtgctggg caacaccgac cggcacagca
      181 tcaagaagaa cctgatcgga gccctgctgt tcgacagcgg cgaaacagcc gaggccaccc
      241 ggctgaagag aaccgccaga agaagataca ccagacggaa gaaccggatc tgctatctgc
      301 aagagatctt cagcaacgag atggccaagg tggacgacag cttcttccac agactggaag
      361 agtccttcct ggtggaagag gataagaagc acgagcggca ccccatcttc ggcaacatcg
      421 tggacgaggt ggcctaccac gagaagtacc ccaccatcta ccacctgaga aagaaactgg
      481 tggacagcac cgacaaggcc gacctgcggc tgatctatct ggccctggcc cacatgatca
      541 agttccgggg ccacttcctg atcgagggcg acctgaaccc cgacaacagc gacgtggaca
      601 agctgttcat ccagctggtg cagacctaca accagctgtt cgaggaa
//
