LOCUS       sequence-139782-         707 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses SpCas9, GFP, and sgRNA targeting Ago1.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 707)
  AUTHORS   Ngondo RP, Cirera-Salinas D, Yu J, Wischnewski H, Bodak M, 
            Vandormael-Pournin S, Geiselmann A, Wettstein R, Luitz J, 
            Cohen-Tannoudji M, Ciaudo C
  TITLE     Argonaute 2 Is Required for Extra-embryonic Endoderm Differentiation
            of Mouse Embryonic Stem Cells.
  JOURNAL   Stem Cell Reports. 2018 Jan 24. pii: S2213-6711(17)30571-4. doi: 
            10.1016/j.stemcr.2017.12.023.
  PUBMED    29396181
REFERENCE   2  (bases 1 to 707)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Stem Cell 
            Reports. 2018 Jan 24. pii: S2213-6711(17)30571-4. doi: 
            10.1016/j.stemcr.2017.12.023."
COMMENT     Sequence Label: pX458-sgRNA_Ago1_3
FEATURES             Location/Qualifiers
     source          1..707
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     102..121
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        201..276
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        373..658
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 ttaatttgac tgtaaacaca aagatattag tacaaaatac gtgacgtaga aagtaataat
       61 ttcttgggta gtttgcagtt ttaaaattat gttttaaaat ggactatcat atgcttaccg
      121 taacttgaaa gtatttcgat ttcttggctt tatatatctt gtggaaagga cgaaacaccg
      181 gagtcaagcg gggcgctcga gttttagagc tagaaatagc aagttaaaat aaggctagtc
      241 cgttatcaac ttgaaaaagt ggcaccgagt cggtgctttt ttgttttaga gctagaaata
      301 gcaagttaaa ataaggctag tccgttttta gcgcgtgcgc caattctgca gacaaatggc
      361 tctagaggta cccgttacat aacttacggt aaatggcccg cctggctgac cgcccaacga
      421 cccccgccca ttgacgtcaa tagtaacgcc aatagggact ttccattgac gtcaatgggt
      481 ggagtattta cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtac
      541 gccccctatt gacgtcaatg acggtaaatg gcccgcctgg cattgtgccc agtacatgac
      601 cttatgggac tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt
      661 cgaggtgagc cccacgttct gcttcactct ccccatctcc cccccct
//
