LOCUS       sequence-139785-         984 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  GoldenBraid level 1 Omega plant expression vector .
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 984)
  AUTHORS   Sarrion-Perdigones A, Vazquez-Vilar M, Palaci J, Castelijns B, 
            Forment J, Ziarsolo P, Blanca J, Granell A, Orzaez D
  TITLE     GoldenBraid 2.0: a comprehensive DNA assembly framework for plant 
            synthetic biology.
  JOURNAL   Plant Physiol. 2013 Jul;162(3):1618-31.
  PUBMED    23669743
REFERENCE   2  (bases 1 to 984)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Plant 
            Physiol."; date: "2013-07"; volume: "162(3)"; pages: "1618-31"
COMMENT     Sequence Label: pDGB1_omega1
FEATURES             Location/Qualifiers
     source          1..984
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     misc_feature    29..53
                     /label=RB T-DNA repeat
                     /note="right border repeat from nopaline C58 T-DNA"
     protein_bind    136..157
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        join(172..177,178..195,196..202)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    210..226
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     215..237
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     primer_bind     234..250
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     234..250
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     misc_feature    263..319
                     /label=MCS
                     /note="pUC18/19 multiple cloning site"
     primer_bind     complement(320..337)
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     complement(320..336)
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(329..351)
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     complement(545..564)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     misc_feature    682..704
                     /label=LB T-DNA repeat
                     /note="left border repeat from nopaline C58 T-DNA 
                     (truncated)"
ORIGIN
        1 atctcaaaca aacacataca gcgacttagt ttacccgcca atatatcctg tcaggatccg
       61 gtctcatgac agcgtgagac gcagctggca cgacaggttt cccgactgga aagcgggcag
      121 tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacacta
      181 tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
      241 cagctatgac catgattacg ccaagcttgc atgcctgcag gtcgactcta gaggatcccc
      301 gggtaccgag ctcgaattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
      361 gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
      421 aagaggcctg cgccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgcc
      481 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
      541 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
      601 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgcgtc
      661 tcactcctga gaccggatcc gttacaccac aatatatcct gccaagatct aattccgggg
      721 atcggaaatc cagaagcccg agaggttgcc gcctttcggg ctttttcttt ttcaaaaaaa
      781 aaaatttata aaacgatctg ttgcggccgg ccgccgggtt gtgggcaaag gcgctcgacg
      841 gtgggcaacc gcttgcggtt gtccacgggc ggagccggtg cgcgtagcgc attgtccaca
      901 agccaagggc gaccaataat tgatatatat attcataatt gaaaagctaa ttgaacatac
      961 tacttgctgt aactacttgc cgga
//
