LOCUS       sequence-139793-         938 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  natural linear DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 938)
  AUTHORS   Scheibel T, Lindquist SL
  TITLE     The role of conformational flexibility in prion propagation and 
            maintenance for Sup35p.
  JOURNAL   Nat Struct Biol 2001 Nov;8(11):958-62.
  PUBMED    11685242
REFERENCE   2  (bases 1 to 938)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Nat Struct 
            Biol"; date: "2001-11"; volume: "8(11)"; pages: "958-62"
COMMENT     Sequence Label: pJC25NM
FEATURES             Location/Qualifiers
     source          1..938
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     RBS             16..38
                     /note="efficient ribosome binding site from bacteriophage 
                     T7 gene 10 (Olins and Rangwala, 1989)"
     primer_bind     complement(879..897)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
ORIGIN
        1 ccctctagaa ataattttgt ttaactttaa gaaggagata tacatatgtc ggattcaaac
       61 caaggcaaca atcagcaaaa ctaccagcaa tacagccaga acggtaacca acaacaaggt
      121 aacaacagat accaaggtta tcaagcttac aatgctcaag cccaacctgc aggtgggtac
      181 taccaaaatt accaaggtta ttctgggtac caacaaggtg gctatcaaca gtacaatccc
      241 gacgccggtt accagcaaca gtataatcct caaggaggct atcaacagta caatcctcaa
      301 ggcggttatc agcagcaatt caatccacaa ggtggccgtg gaaattacaa aaacttcaac
      361 tacaataaca atttgcaagg atatcaagct ggtttccaac cacagtctca aggtatgtct
      421 ttgaacgact ttcaaaagca acaaaagcag gccgctccca aaccaaagaa gactttgaag
      481 cttgtctcca gttccggtat caagttggcc aatgctacca agaaggttgg cacaaaacct
      541 gccgaatctg ataagaaaga ggaagagaag tctgctgaaa ccaaagaacc aactaaagag
      601 ccaacaaagg tcgaagaacc agttaaaaag gaggagaaac cagtccagac tgaagaaaag
      661 acggaggaaa aatcggaact tccaaaggta gaagacctta aaatctctga atcaacacat
      721 aataccaaca atgccaatgt taccagtgct gatgccttga tcaaggaaca ggaagaagaa
      781 gtggatgacg aagttgttaa cgattaagga tcccgggccc tagctaactg atccggctgc
      841 taacaaagcc cgaaaggaag ctgagttggc tgctgccacc gctgagcaat aactagcata
      901 accccttggg gcctctaaac gggtcttgag gggttttt
//
