LOCUS       sequence-139795-         756 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4E) mCherry 
            Non functional sensor with phospho-mimicking NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 756)
  AUTHORS   Durandau E, Aymoz D, Pelet S
  TITLE     Dynamic single cell measurements of kinase activity by synthetic 
            kinase activity relocation sensors.
  JOURNAL   BMC Biol. 2015 Aug 1;13:55. doi: 10.1186/s12915-015-0163-z.
  PUBMED    26231587
REFERENCE   2  (bases 1 to 756)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2015-08-1"; volume: "13:55. doi"; pages: " 
            10.1186/s12915-015-0163-z"
COMMENT     Sequence Label: pED54pRS306 pRPS2-Ste7DS NLS(4E)-mCherry
FEATURES             Location/Qualifiers
     source          1..756
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 attcccactt ttactatgct tcaattgacc cttcggtatc tcagcatcta ggtagctttc
       61 attgagattc atagttccga tgtactgatt ctacgtttgt gtactttgat agactttttt
      121 attcaattcg tattcactac tatctttgtc tcgtaccgcg gtttttatcc tttatttcag
      181 ccctacgaga tagaacgatg gagcctagta ataccatttt atgtaagcaa aataagaaga
      241 aagactaaaa gaattaggat atgttgttaa tcagggataa aggtacgcat actagcctta
      301 ctacgtaaat ataaggttac ggtttacgat cactattgca agatactatt gtttagcatt
      361 ggcaaagact tgacgggaaa tctttgattg ggcccaacca tattcattaa agatccggga
      421 tgtgagcttg aactgctttt cgacgcgaag aaggtttaca cgtattttag taattggctt
      481 attcactaag gattcttaag gttttcttaa tagttttcta cgtcggcatg cgattgtttg
      541 gtttagaaga ctgctttcta aatatggttg ggtgtattta agctagaccc atacacccgc
      601 tctatgggat tatttacttg tttgaatttt aagatttgtg ataatggaac tggacgcaaa
      661 catttgatgg aaaacgcatg tcatcattaa cgaggtaacg taggtatctg tcctgcctta
      721 gtattgcacg cagcttccca ggacgcctag ctattt
//
