LOCUS       sequence-139817-         548 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  FlAsH-BRET reporter to monitor conformational changes in Arrestin3. 
            Insertion of FlAsH motif (CCPGCC) following amino acid residue 171 
            of Arrestin3..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 548)
  AUTHORS   Lee MH, Appleton KM, Strungs EG, Kwon JY, Morinelli TA, Peterson YK,
            Laporte SA, Luttrell LM
  TITLE     The conformational signature of beta-arrestin2 predicts its 
            trafficking and signalling functions.
  JOURNAL   Nature. 2016 Mar 31;531(7596):665-8. doi: 10.1038/nature17154. Epub 
            2016 Mar 23.
  PUBMED    27007854
REFERENCE   2  (bases 1 to 548)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1038/nature17154"; journalName: "Nature"; date: "2016-03-31- 
            31"; volume: "531"; issue: "7596"; pages: "665-8"
COMMENT     Sequence Label: Rlucrarr3-Flash3
FEATURES             Location/Qualifiers
     source          1..548
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(8..24)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8..24)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(21..43)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    32..48
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(56..62,63..80,81..86))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    101..122
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(239..256)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     primer_bind     complement(490..509)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
       61 tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
      121 taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
      181 aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
      241 cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
      301 aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
      361 aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc
      421 tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
      481 caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
      541 cgaccctg
//
