LOCUS       sequence-139829-         968 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  BFPRKAB_PUFa in pCR8 gateway donor vector.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 968)
  AUTHORS   Cheng AW, Jillette N, Lee P, Plaskon D, Fujiwara Y, Wang W, 
            Taghbalout A, Wang H
  TITLE     Casilio: a versatile CRISPR-Cas9-Pumilio hybrid for gene regulation 
            and genomic labeling.
  JOURNAL   Cell Res. 2016 Feb;26(2):254-7. doi: 10.1038/cr.2016.3. Epub 2016 
            Jan 15.
  PUBMED    26768771
REFERENCE   2  (bases 1 to 968)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: "10.1038/cr.2016.3";
            journalName: "Cell Res"; date: "2016-02"; volume: "26"; issue: "2"; 
            pages: "254-7"
COMMENT     Sequence Label: pAC1450_pCR8-BFPKRAB_PUFa
FEATURES             Location/Qualifiers
     source          1..968
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     protein_bind    42..66
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     regulatory      84..93
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             96..122
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             102..128
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /note="This is a variant of the standard HA tag, which has 
                     sequence YPYDVPDYA. Some anti-HA antibodies may not 
                     recognize this variant."
                     /translation="YDVPDYASL"
     CDS             141..161
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             168..188
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     CDS             228..923
                     /codon_start=1
                     /product="monomeric blue fluorescent protein"
                     /label=TagBFP
                     /note="mammalian codon-optimized"
                     /translation="SELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKVV
                     EGGPLPFAFDILATSFLYGSKTFINHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTAT
                     QDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALKL
                     VGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYCD
                     LPSKLGHKLN"
ORIGIN
        1 gttcgttgca acaaattgat gagcaatgct tttttataat gccaactttg tacaaaaaag
       61 caggctccga attcaccggt gccgccacca tggcctatcc ctatgacgtg cccgattatg
      121 ccagcctggg cagcggctcc cccaagaaaa aacgcaaggt ggaagatcct aagaaaaagc
      181 ggaaagtgga cggcattggt agtgggagca acggcagcag cggatccagc gagctgatta
      241 aggagaacat gcacatgaag ctgtacatgg agggcaccgt ggacaaccat cacttcaagt
      301 gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga atcaaggtgg
      361 tcgagggcgg ccctctcccc ttcgccttcg acatcctggc tactagcttc ctctacggca
      421 gcaagacctt catcaaccac acccagggca tccccgactt cttcaagcag tccttccctg
      481 agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctg accgctaccc
      541 aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga ggggtgaact
      601 tcacatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc ttcaccgaga
      661 cgctgtaccc cgctgacggc ggcctggaag gcagaaacga catggccctg aagctcgtgg
      721 gcgggagcca tctgatcgca aacatcaaga ccacatatag atccaagaaa cccgctaaga
      781 acctcaagat gcctggcgtc tactatgtgg actacagact ggaaagaatc aaggaggcca
      841 acaacgagac ctacgtcgag cagcacgagg tggcagtggc cagatactgc gacctcccta
      901 gcaaactggg gcacaagctt aatggcggtg gcggagggat ggatgctaag tcactaactg
      961 cctggtcc
//
