LOCUS       sequence-139837-         702 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Expresses a human codon-optimized SpCas9 and sgRNA targeting Dgcr8.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 702)
  AUTHORS   Cirera-Salinas D, Yu J, Bodak M, Ngondo RP, Herbert KM, Ciaudo C
  TITLE     Noncanonical function of DGCR8 controls mESC exit from pluripotency.
  JOURNAL   J Cell Biol. 2017 Jan 18. pii: jcb.201606073. doi: 
            10.1083/jcb.201606073.
  PUBMED    28100686
REFERENCE   2  (bases 1 to 702)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "J Cell 
            Biol. 2017 Jan 18. pii: jcb.201606073. doi: 10.1083/jcb.201606073."
COMMENT     Sequence Label: pX330-sgRNA_Dgcr8_2
FEATURES             Location/Qualifiers
     source          1..702
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     103..122
                     /label=LKO.1 5'
                     /note="Human U6 promoter, forward primer"
     misc_RNA        200..275
                     /label=gRNA scaffold
                     /note="guide RNA scaffold for the Streptococcus pyogenes 
                     CRISPR/Cas9 system"
     enhancer        372..657
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer; 
                     contains an 18-bp deletion relative to the standard CMV 
                     enhancer"
ORIGIN
        1 attaatttga ctgtaaacac aaagatatta gtacaaaata cgtgacgtag aaagtaataa
       61 tttcttgggt agtttgcagt tttaaaatta tgttttaaaa tggactatca tatgcttacc
      121 gtaacttgaa agtatttcga tttcttggct ttatatatct tgtggaaagg acgaaacacc
      181 gcatagaccc gaactgtagg ttttagagct agaaatagca agttaaaata aggctagtcc
      241 gttatcaact tgaaaaagtg gcaccgagtc ggtgcttttt tgttttagag ctagaaatag
      301 caagttaaaa taaggctagt ccgtttttag cgcgtgcgcc aattctgcag acaaatggct
      361 ctagaggtac ccgttacata acttacggta aatggcccgc ctggctgacc gcccaacgac
      421 ccccgcccat tgacgtcaat agtaacgcca atagggactt tccattgacg tcaatgggtg
      481 gagtatttac ggtaaactgc ccacttggca gtacatcaag tgtatcatat gccaagtacg
      541 ccccctattg acgtcaatga cggtaaatgg cccgcctggc attgtgccca gtacatgacc
      601 ttatgggact ttcctacttg gcagtacatc tacgtattag tcatcgctat taccatggtc
      661 gaggtgagcc ccacgttctg cttcactctc cccatctccc cc
//
