LOCUS       sequence-139839-         885 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Green fluorescent reporter of Orai1-associated calcium influx.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 885)
  AUTHORS   Dynes JL, Amcheslavsky A, Cahalan MD
  TITLE     Genetically targeted single-channel optical recording reveals 
            multiple Orai1 gating states and oscillations in calcium influx.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jan 12;113(2):440-5. doi: 
            10.1073/pnas.1523410113. Epub 2015 Dec 28.
  PUBMED    26712003
REFERENCE   2  (bases 1 to 885)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1523410113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-01-12- 12"; volume: "113"; issue: "2"; pages: "440-5"
COMMENT     Sequence Label: G-GECO1-Orai1
FEATURES             Location/Qualifiers
     source          1..885
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      31..40
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             52..108
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     321..342
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     primer_bind     complement(709..728)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
ORIGIN
        1 gaaccgtcag atccgctagc gctaccggtc gccaccatgg tcgactcatc acgtcgtaag
       61 tggaataaga caggtcacgc agtcagagct ataggtcggc tgagctcact cgagaacgtc
      121 tatatcaagg ccgacaagca gaagaacggc atcaaggcga acttcaagat ccgccacaac
      181 atcgaggacg gcggcgtgca gctcgcctac cactaccagc agaacacccc catcggcgac
      241 ggccccgtgc tgctgcccga caaccactac ctgagcgtgc agtccatact ttcgaaagac
      301 cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc cgggatcact
      361 ctcggcatgg acgagctgta caagggcggt accggaggga gcatggtgag caagggcgag
      421 gagctgttca ccggggtggt gcccatccag gtcgagctgg acggcgacgt aaacggccac
      481 aagttcagcg tgtccggcga gggtgagggc gatgccacct acggcaagct gaccctgaag
      541 ttcatctgca ccaccggcaa gctgcccgtg ccctggccca ccctcgtgac caccctgacc
      601 tacggcgtgc agtgcttcag ccgctacccc gaccacatga agcagcacga cttcttcaag
      661 tccgccatgc ccgaaggcta catccaggag cgcaccatct tcttcaagga cgacggcaac
      721 tacaagaccc gcgccgaggt gaagttcgag ggcgacaccc tggtgaaccg catcgagctg
      781 aagggcatcg acttcaagga ggacggcaac atcctggggc acaagctgga gtacaacacg
      841 cgtgaccaac tgactgaaga gcagatcgca gaatttaaag aggct
//
