LOCUS       sequence-139840-         544 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  promoterless Tol2 vector with mCherry tag upstream of and in frame 
            with attR1/attR2 cassette and late SV40 polyadenylation signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 544)
  AUTHORS   Villefranc JA, Amigo J, Lawson ND
  TITLE     Gateway compatible vectors for analysis of gene function in the 
            zebrafish.
  JOURNAL   Dev Dyn. 2007 Nov . 236(11):3077-87.
  PUBMED    17948311
REFERENCE   2  (bases 1 to 544)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2007 Nov . 236(11):3077-87."
COMMENT     Sequence Label: 366 pTol2-CherryDest
FEATURES             Location/Qualifiers
     source          1..544
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     136..159
                     /label=DsRed1-C
                     /note="DsRed1, forward primer"
     protein_bind    249..373
                     /gene="mutant version of attR"
                     /label=attR1
                     /bound_moiety="LR Clonase(TM)"
                     /note="recombination site for the Gateway(R) LR reaction"
     promoter        join(398..403,404..421,422..428)
                     /label=lac UV5 promoter
                     /note="E. coli lac promoter with an ""up"" mutation"
ORIGIN
        1 aggacggcgc cctgaagggc gagatcaagc agaggctgaa gctgaaggac ggcggccact
       61 acgacgccga ggtcaagacc acctacaagg ccaagaagcc cgtgcagctg cccggcgcct
      121 acaacgtcaa catcaagctg gacatcacct cccacaacga ggactacacc atcgtggaac
      181 agtacgagcg cgccgagggc cgccactcca ccggcggcat ggacgagctg tacaagagat
      241 ctcgaatcac aagtttgtac aaaaaagctg aacgagaaac gtaaaatgat ataaatatca
      301 atatattaaa ttagattttg cataaaaaac agactacata atactgtaaa acacaacata
      361 tccagtcact atggcggccg cattaggcac cccaggcttt acactttatg cttccggctc
      421 gtataatgtg tggattttga gttaggatcc gtcgagattt tcaggagcta aggaagctaa
      481 aatggagaaa aaaatcactg gatataccac cgttgatata tcccaatggc atcgtaaaga
      541 acat
//
