LOCUS       sequence-139841-         810 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  SKARS: Fus3 and Kss1 MAPK activity sensor: Ste7DS 2xNLS(4E) mCherry 
            Non functional sensor with phospho-mimicking NLS.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 810)
  AUTHORS   Durandau E, Aymoz D, Pelet S
  TITLE     Dynamic single cell measurements of kinase activity by synthetic 
            kinase activity relocation sensors.
  JOURNAL   BMC Biol. 2015 Aug 1;13:55. doi: 10.1186/s12915-015-0163-z.
  PUBMED    26231587
REFERENCE   2  (bases 1 to 810)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "BMC Biol.";
            date: "2015-08-1"; volume: "13:55. doi"; pages: " 
            10.1186/s12915-015-0163-z"
COMMENT     Sequence Label: pED54pRS306 pRPS2-Ste7DS NLS(4E)-mCherry
FEATURES             Location/Qualifiers
     source          1..810
                     /mol_type="genomic DNA"
                     /organism="unspecified"
ORIGIN
        1 cctcgatctc gaactcgtgg ccgttcacgg agccctccat gtgcaccttg aagcgcatga
       61 actccttgat gatggccatg ttatcctcct cgcccttgct agcagatcta accaaaccag
      121 aagccgtatc tggttctatt tttagttttt ttagcggctc acctagttct tcaaattccg
      181 aaccacgacc catttgttgg aattcaacca aaccagaagc cgtatctggt tctattttta
      241 gtttttttag cggctcacct agttcttcaa attccgaacc acgacccatt tgttgtctag
      301 atgtcttttc ctgcaattgg ccattattgc ccacatctgg gtgcaggtta agattgagcc
      361 ctttcaagtt ccttctctgt aaagtctttc gttgaaacat aagctttact taattagttg
      421 agcttattgg ttgtttgtta tagtcggaat ggaacacgga aagttgatgt aggtaacttt
      481 caatcgacaa tatataaaga acacggtttc tttgggattg aaaaggtatc aagcagtaag
      541 gaaggaaatt ggaaacaaag gaagagaaag tctgaattaa aaacttgaaa actcttacgt
      601 tactacagag gggaatagat gaaaaaatag ctaggcgtcc tgggaagctg cgtgcaatac
      661 taaggcagga cagataccta cgttacctcg ttaatgatga catgcgtttt ccatcaaatg
      721 tttgcgtcca gttccattat cacaaatctt aaaattcaaa caagtaaata atcccataga
      781 gcgggtgtat gggtctagct taaatacacc
//
