LOCUS       sequence-139867-         888 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Green fluorescent reporter of Orai1-associated calcium influx.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 888)
  AUTHORS   Dynes JL, Amcheslavsky A, Cahalan MD
  TITLE     Genetically targeted single-channel optical recording reveals 
            multiple Orai1 gating states and oscillations in calcium influx.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jan 12;113(2):440-5. doi: 
            10.1073/pnas.1523410113. Epub 2015 Dec 28.
  PUBMED    26712003
REFERENCE   2  (bases 1 to 888)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1523410113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-01-12- 12"; volume: "113"; issue: "2"; pages: "440-5"
COMMENT     Sequence Label: G-GECO1.2-Orai1
FEATURES             Location/Qualifiers
     source          1..888
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      41..50
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             62..118
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     331..352
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 ctggtttagt gaaccgtcag atccgctagc gctaccggtc gccaccatgg tcgactcatc
       61 acgtcgtaag tggaataaga caggtcacgc agtcagagct ataggtcggc tgagctcact
      121 cgagaacgtc tatatcaagg ccgacgagca gaagaacggc atcaaggcgt acttcaagat
      181 ccgccacaac atcgagggcg gcggcgtgca gctcgcctac cactaccagc agaacacccc
      241 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcgtgc agtccatgct
      301 ttcgaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
      361 cgggatcact ctcggcatgg acgagctgta caagggcggt accggaggga gcgaatccat
      421 ggtgagcaag ggcgaggagc tgttcaccgg ggtggtgccc atccaggtcg agctggacgg
      481 cgacgtaaac ggccacaagt tcagcgtgtc cggcgagggt gagggcgatg ccacctacgg
      541 caagctgacc ctgaagttca tctgcaccac cggcaagctg cccgtgccct ggcccaccct
      601 cgtgaccacc ctgacctacg gcgtgcagtg cttcagccgc taccccgacc acatgaagca
      661 gcacgacttc ttcaagtccg ccatgcccga aggctacatc caggagcgca ccatcttctt
      721 caagggcgac ggcaactaca agacccgcgc cgaggtgaag ttcgagggcg acaccctggt
      781 gaaccgcatc gagctgaagg gcatcgactt caaggaggac ggcaacatcc tggggcacaa
      841 gctggagtac aacacgcgtg accaactgac tgaagagcag atcgcaga
//
