LOCUS       sequence-139878-         914 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Green fluorescent reporter of Orai3-associated calcium influx.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 914)
  AUTHORS   Dynes JL, Amcheslavsky A, Cahalan MD
  TITLE     Genetically targeted single-channel optical recording reveals 
            multiple Orai1 gating states and oscillations in calcium influx.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jan 12;113(2):440-5. doi: 
            10.1073/pnas.1523410113. Epub 2015 Dec 28.
  PUBMED    26712003
REFERENCE   2  (bases 1 to 914)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1523410113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-01-12- 12"; volume: "113"; issue: "2"; pages: "440-5"
COMMENT     Sequence Label: G-GECO1-Orai3
FEATURES             Location/Qualifiers
     source          1..914
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     primer_bind     complement(23..42)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     CDS             158..598
                     /codon_start=1
                     /product="calcium-binding protein"
                     /label=calmodulin
                     /translation="DQLTEEQIAEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEA
                     ELQDMINEVDADGDGTIDFPEFLTMMARKMKDTDSEEEIREAFRVFDKDGNGYIGAAEL
                     RHVMTNLGEKLTDEEVDEMIRVADIDGDGQVNYEEFVQMMTAK"
     regulatory      846..855
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
ORIGIN
        1 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
       61 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
      121 caacatcctg gggcacaagc tggagtacaa cacgcgtgac caactgactg aagagcagat
      181 cgcagaattt aaagaggctt tctccctatt tgacaaggac ggggatggga cgataacaac
      241 caaggagctg gggacggtga tgcggtctct ggggcagaac cccacagaag cagagctgca
      301 ggacatgatc aatgaagtag atgccgacgg tgacggcaca atcgacttcc ctgagttcct
      361 gacaatgatg gcaagaaaaa tgaaagacac agacagtgaa gaagaaatta gagaagcgtt
      421 ccgtgtgttt gataaggacg gcaatggcta catcggcgca gcagagcttc gccacgtgat
      481 gacaaacctt ggagagaagt taacagatga agaggttgat gaaatgatca gggtagcaga
      541 catcgatggg gatggtcagg taaactacga agagtttgta caaatgatga cagcgaagca
      601 gatctcgagc tcaagcttcg aattcaaggg cggcgagggg gacgcgggcg agcaggcccc
      661 gctgaaccct gagggcgaga gccctgcagg ctcggccacg taccgggagt tcgtgcaccg
      721 cggctacctg gacctcatgg gggccagtca gcactcgctg cgggcgctca gctggcgccg
      781 cctctacctc agccgggcca agctcaaagc ttccagccgc acgtctgcct tgctctcggg
      841 cttcgccatg gtggccatgg tggaggtgca gctggagagt gaccacgagt acccaccagg
      901 cctgctggtg gcct
//
