LOCUS       sequence-139884-        1047 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Produces Acetobacter aceti 1023 N5-carboxyaminoimidazole 
            ribonucleotide mutase with N-terminal His6 tag (H6AaPurE1).
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 1047)
  AUTHORS   Kappock
  TITLE     Additional purEK associated constructs
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 1047)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: 
            "Unpublished"
COMMENT     Sequence Label: pJK554
FEATURES             Location/Qualifiers
     source          1..1047
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     CDS             49..66
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     CDS             76..93
                     /codon_start=1
                     /product="thrombin recognition and cleavage site"
                     /label=thrombin site
                     /translation="LVPRGS"
     CDS             695..739
                     /codon_start=1
                     /product="affinity and epitope tag derived from pancreatic 
                     ribonuclease A"
                     /label=S-Tag
                     /translation="KETAAAKFERQHMDS"
     primer_bind     complement(777..795)
                     /label=T7 Term
                     /note="T7 terminator, reverse primer"
     terminator      791..838
                     /label=T7 terminator
                     /note="transcription terminator for bacteriophage T7 RNA 
                     polymerase"
ORIGIN
        1 aaataatttt gtttaacttt aataaggaga tataccatgg gcagcagcca tcatcatcat
       61 catcacagca gcggcctggt gccgcgcggc agccatatga tgagcgaaac cgcccccctg
      121 ccctctgctt ccagtgcttt agaagataaa gcagcctctg cccccgttgt gggcattatc
      181 atgggcagcc agtcagactg ggaaaccatg cgccatgcag acgcattgct gacagagctg
      241 gaaattccgc atgaaaccct cattgtttca gcacatcgta cgccagaccg gttggcggat
      301 tatgcgcgta cggcagccga acgcggcctg aatgtgatta ttgctggggc cggtggagct
      361 gcacatctgc cgggtatgtg tgcggcatgg acacgcctgc ccgtgctggg tgttccagta
      421 gaatctcgcg ctcttaaagg catggacagc ctgctttcca ttgtgcagat gcccggcggc
      481 gtgcctgtgg gcactttggc tattggggca tccggcgcca aaaatgccgc cttgctggcc
      541 gcctccattc ttgcactgta taacccggcc cttgccgcac gccttgaaac atggcgcgca
      601 ttgcagacgg cctctgtccc caactctccc attaccgaag ataaatgaga attcgagctc
      661 cgtcgacaag cttgcggccg cactcgagtc tggtaaagaa accgctgctg cgaaatttga
      721 acgccagcac atggactcgt ctactagcgc agcttaatta acctaggctg ctgccaccgc
      781 tgagcaataa ctagcataac cccttggggc ctctaaacgg gtcttgaggg gttttttgct
      841 gaaacctcag gcatttgaga agcacacggt cacactgctt ccggtagtca ataaaccggt
      901 aaaccagcaa tagacataag cggctattta acgaccctgc cctgaaccga cgaccgggtc
      961 atcgtggccg gatcttgcgg cccctcggct tgaacgaatt gttagacatt atttgccgac
     1021 taccttggtg atctcgcctt tcacgta
//
