LOCUS       sequence-139889-         921 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Green fluorescent reporter of mutant Orai1Y80E-associated calcium 
            influx.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 921)
  AUTHORS   Dynes JL, Amcheslavsky A, Cahalan MD
  TITLE     Genetically targeted single-channel optical recording reveals 
            multiple Orai1 gating states and oscillations in calcium influx.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jan 12;113(2):440-5. doi: 
            10.1073/pnas.1523410113. Epub 2015 Dec 28.
  PUBMED    26712003
REFERENCE   2  (bases 1 to 921)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1523410113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-01-12- 12"; volume: "113"; issue: "2"; pages: "440-5"
COMMENT     Sequence Label: G-GECO1.2-Orai1Y80E
FEATURES             Location/Qualifiers
     source          1..921
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      37..46
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             58..114
                     /codon_start=1
                     /product="calmodulin-binding peptide from avian smooth 
                     muscle myosin light chain kinase"
                     /label=calmodulin-binding peptide
                     /translation="RRKWNKTGHAVRAIGRLSS"
     primer_bind     327..348
                     /label=EGFP-C
                     /note="EGFP, forward primer"
ORIGIN
        1 tttagtgaac cgtcagatcc gctagcgcta ccggtcgcca ccatggtcga ctcatcacgt
       61 cgtaagtgga ataagacagg tcacgcagtc agagctatag gtcggctgag ctcactcgag
      121 aacgtctata tcaaggccga cgagcagaag aacggcatca aggcgtactt caagatccgc
      181 cacaacatcg agggcggcgg cgtgcagctc gcctaccact accagcagaa cacccccatc
      241 ggcgacggcc ccgtgctgct gcccgacaac cactacctga gcgtgcagtc catgctttcg
      301 aaagacccca acgagaagcg cgatcacatg gtcctgctgg agttcgtgac cgccgccggg
      361 atcactctcg gcatggacga gctgtacaag ggcggtaccg gagggagcga atccatggtg
      421 agcaagggcg aggagctgtt caccggggtg gtgcccatcc aggtcgagct ggacggcgac
      481 gtaaacggcc acaagttcag cgtgtccggc gagggtgagg gcgatgccac ctacggcaag
      541 ctgaccctga agttcatctg caccaccggc aagctgcccg tgccctggcc caccctcgtg
      601 accaccctga cctacggcgt gcagtgcttc agccgctacc ccgaccacat gaagcagcac
      661 gacttcttca agtccgccat gcccgaaggc tacatccagg agcgcaccat cttcttcaag
      721 ggcgacggca actacaagac ccgcgccgag gtgaagttcg agggcgacac cctggtgaac
      781 cgcatcgagc tgaagggcat cgacttcaag gaggacggca acatcctggg gcacaagctg
      841 gagtacaaca cgcgtgacca actgactgaa gagcagatcg cagaatttaa agaggctttc
      901 tccctatttg acaaggacgg g
//
