LOCUS       sequence-139894-       11514 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the Sdk2 protein, C-terminally
            fused to the Fc region of human IgG1 + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11514)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 11514)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Sdk2-Fc-His
FEATURES             Location/Qualifiers
     source          1..11514
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             8093..8110
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    8137..8258
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8174..8193)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8228..8247
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8296..8313)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8467..9055)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8547..8566)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9226..10017,10018..10086))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9849..9868
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10087..10191)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10259..10277
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10315..10337)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10339..10359)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10344..10674
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10525..10660
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10587..10606
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     10811..10830
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(10897..11352)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11034..11055)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11246..11265
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11478..11500
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11492..11509
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11493..11509
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tgtggaggct
     1621 gccgctctgg actctactgg ccctgcatcg gatccactcg gccggggccc aagatgatgt
     1681 accgccatac ttcaagacgg agccagtgcg cacacaagtg catctggaag ggaaccgcct
     1741 ggtgctcacc tgcatggctg aaggcagctg gccgctggag ttcaagtggc tacacaacaa
     1801 tcgggagctg acccggttct cgctggagta caggtacatg attaccagcc tggaccgcac
     1861 ccacgctggc ttctaccgct gcatcgtgcg taaccgaatg ggggccctgc tacagcggca
     1921 gactgaagtc caggtggcct acatggggag ctttgaggaa ggcgagaagc gccaaagtgt
     1981 gaaccatgga gaagctgctg tcatcagagc acctcgcatt tccagcttcc cccggccaca
     2041 ggtgacctgg ttccgtgacg gacgcaagat cccacccagc agccgcatag ccatcaccct
     2101 ggagaacacc ctggtcatcc tgtcgacggt ggctcccgat gctggccgct actacgtgca
     2161 ggcggttaat gacaagaacg gagacaacaa gaccagccag cccatcactc ttgccgtgga
     2221 gaatgttggg ggacccgctg accccattgc accaaccatc atcatcccac ccaagaatac
     2281 cagtgtcgtg gctgggacct cggaggtgac catggagtgt gtggccaacg ccaggcctct
     2341 catcaagctg cacattgtgt ggaagaagga cggggctcca ctctccagtg gcatcagcga
     2401 ctacaaccgc aggctcacca tcgccaaccc cacggtcagc gacgccggct actacgagtg
     2461 cgaagccatg ctgcgcagca gcagcgtggc ccctgtcacc cggggcgcct acctctcggt
     2521 gctggagcca cctcagtttg tcagagagcc tgagagacat atcacggccg agatggaaaa
     2581 ggtggtggac atcccctgtc gagccaaagg ggtaccgcct ccctccatca cctggtacaa
     2641 ggacgctgcg ttggtggagg tggggaagct aacccgcttc aagcagcgca gcgatggcgg
     2701 cctgcagatc agcggcctgc tgcccgacga caccggcatg ctccagtgct ttgcccacaa
     2761 tgcagctggc gaggcccaga cctccaccta cctggctgtc accagcattg cccctaatat
     2821 caccagaggc ccccttgaca gcacggtgat tgacggcatg tcggtggtac tagcatgtga
     2881 gacctcaggg gcaccacggc ctgctatcac ttggcaaaaa ggggagcgca tcttggccag
     2941 tggctctgtc cagctgcctc gattcacact gctggaatct ggcagcctgc tcattagccc
     3001 aacccacatc tcggacgccg gcacctacac ttgtttggcc accaactctc ggggagtaga
     3061 cgaggcgtca gcagacctgg tggtgtgggc ccggacacgc atcactaagc caccccagga
     3121 ccagagtgtc atcaagggca cccaggcctc catggtgtgt ggagtgaccc acgatcccag
     3181 agtgactgtc aggtacgtgt gggagaagga tggggccacc ctggccgtgg agactaatcc
     3241 tcggatccgc ctggacagaa atggctccct acacatctcg cagacgtggt caggggacat
     3301 tggcacgtat acctgccggg tgctctcggc tggtggcaat gactctcgca acgcccacct
     3361 gcgagtcagg cagctccccc atgcccctga gcaccctgtg gcaaccctca gcaccgtgga
     3421 gagacgcgcc atcaacctga cctgggccaa acccttcgac ggcaacagcc ctctgatgcg
     3481 ctacgtcttg gagatgtcag aaaacaatgc tccctggacc atactcctgg ccagcgtgga
     3541 cccagaagct acctcagtga tggtcaaggg actggttcct gctcgatcct accagttccg
     3601 cctgtgcgct gtcaatgatg tgggcaaagg acagttcagt aaggacacag aaagggtgtc
     3661 ccttcctgag gagcccccca cggcccctcc gcagaacgtc atcgccagcg gccggaccaa
     3721 ccaatccatc atgatccaat ggcaaccacc tcccgagagc caccagaatg ggatcctcaa
     3781 gggctacatc atcagatact gcctggcagg gctacctgtt gggtaccaat ttaagaacat
     3841 cacagatgct gacgtcaaca acctgttgct ggaggacctc atcatatgga ccaattacga
     3901 gatcgaggtg gctgcctaca acagcgccgg gctgggtgtc tacagcagca aagtcaccga
     3961 gtggacgctg cagggagttc ccacggtgcc cccgggcaat gtgcatgcag aagccacaaa
     4021 ttctacaacc atccgattca cctggaatgc cccaagcccg cagttcatca acggcatcaa
     4081 ccagggctac aagctgattg cctgggagcc agcccaagaa gaggaggtta ccatggtgac
     4141 cgccagaccc aactttcaag acagcatcca tgtgggcttc gtgtccggcc tgaaaaaatt
     4201 cacagagtac tttacctcgg tcctctgctt caccacaccg ggagacgggc ctcggagcag
     4261 cccccagctg gtgcgcacac acgaggacgt acctggaccc gtgggacacc tgagcttcaa
     4321 tgacatcctg gacacctcgc tgaaggtcag ctggcaggag ccaggcgaga agaacggcat
     4381 cctgacaggg taccggatct cctgggaaga gtacaacaga accaacaccc gtgtgaccca
     4441 ctacctgccc aacgtaaccc tggagtaccg agtcacgggc ctcacagcac tcactaccta
     4501 tactatagag gtggccgcca tgacctccaa gggccagggc caggtgtcgg cctccactat
     4561 ttcatctggt gtgcctccag agcttccagg ggcacccacc aacctgggca tttccaacat
     4621 cggcccacga tccgtgacct tgcagttcag gcctggctat gacgggaaga cgtccatctc
     4681 ccgctgggtg gtggaggctc aggtgggcgt gatcggggag ggcgaggagt ggttgctcat
     4741 ctaccaactc ggcaatgagc ctgacgcccg ttccatggag gtgccggacc tcaacccctt
     4801 cacctactac agcttccgca tgcgccaagt gaacatcgtt ggcaccagcc cacccagtca
     4861 accttctaga aagatccaga ccctccaggc gcccccagac atagctccag ccaacgtgac
     4921 tctgcgcaca gccagtgaga ccagcttgtg gctccgatgg atgcccctcc cagaaatgga
     4981 atacaacggc aaccctgagt cggtgggcta caagatcaag tacggccgct ctgacggcca
     5041 cggcaagacg ctgagccaca cggtacaaga ccgagtggag cgagaataca ctattgagga
     5101 cctggaggag tggacagagt accgcgtcca agttcaggcc ttcaacgcca tcgggagcgg
     5161 gccgtggagc caggcagtgg tgggcaggac ccgggagtca gtcccatcct ctggccccac
     5221 caacgtgtcg gctctggcca ccacctctag cagcatgctg gtccgctgga gcgagatccc
     5281 ggaggcagat cgaaatggcc tcgtgctggg ctacaaggtg aggtacaagg agaaggactc
     5341 ggactcccag ccccggttct ggctggtaga aggcaactca tcccgcagcg cccagctcac
     5401 aggcctgggc aagtatgtgc tctacgaggt ccaggttctg gccttcacac gcatcggcga
     5461 cggcagcccg agccaccctc ccatcctgga gcggacacta gatgatgtcc caggcccccc
     5521 catgggcatc ctgtttccag aggtgagaac cacatctgtg cggctcatct ggcagccccc
     5581 agcagctccc aacggcatca ttctggccta ccagatcaca caccgtctca acgccactac
     5641 agccaacacc gccacagtgg aggtcctggc ccccagcgcc cgccagtaca tggccacagg
     5701 cctcaagccg gagtctgtct acctattccg tatcacagcc caaacccgca aaggctgggg
     5761 agaggctgct gaggccttgg tggtgactac agagaagaga gaccggccgc agccccctag
     5821 caagcccgtg gtacagcagg aagacgtgaa agcacgcagt gtgctgctgt cctgggaacc
     5881 aggcagcgat gggctctccc cagtacgcta ctacaccatc cagacgcgcg agctgcccag
     5941 cggcaggtgg gcgctgcact cggcctccgt gagccacaat gcctccgcct tcactgtgga
     6001 cagactcaaa ccctttacgt cctacaagtt ccgagtgaag gcgaccaacg acatcggcga
     6061 cagcgagttc agcgaggaat cggagtcgct gaccaccctg caggccgccc cagacgaggc
     6121 acctaccatc ctgtcagtga ctccacacac caccacctct gtgctaatcc gatggcagcc
     6181 tccatctgag gacaagatca acggcatcct cttgggcttc cggatccggt accgggagct
     6241 gctctacgat gggctgaggg gcttcactct tagaggcatc aacaaccctg gggccaagtg
     6301 ggctgagctc acctccttgt actccatgcg gaacctgacc cggcccagcc tcacgcagta
     6361 cgagctggac aatctgagca agcacaggcg ttacgagatc cgaatgagca tatacaacgc
     6421 cgtgggtgag ggacccttga gcccgccgca agaggtcttt gttggggagg cagtgcccac
     6481 ggcagcaccg cagaacgtgg ccatccacag tgccacagcc acacagctgg atgtgacctg
     6541 ggagccaccc cctttggaca accagaatgg agacatccaa ggatacaaga tttatttctg
     6601 ggaagtccaa cggaggaacc tcacagagag ggtgaagact ctcttcttgg ctgagaacag
     6661 cgtgaaactc aagaacctga cgggctacac agcctacatg gtcagcgtgg cagccttcaa
     6721 tgctgcgggg gatgggccga ggagcacccc gacccggggc cagactcagc aagcagcccc
     6781 cagtgccccg ggctcggtca agttcagcga gctgaccact acctcagtga atgtgtcctg
     6841 ggatgcccca cagttcccca acggtcctct ggagggctac aggctggtgt acgaaccctg
     6901 caccccagtg gatggggtca gcaagattgt gacagtggac gtgaagggga acagcccact
     6961 gtggctgaaa gtgaaggatc tggccgaggg catgacgtat cgtttccgaa tcaaagccaa
     7021 gaccttcacc tatgggcctg agattgaagc caacatcact acgggccctg gagaaggtgc
     7081 cccaggacca ccgggagtac cgatcatcgt tcgctacagc tcagccattg ccatccactg
     7141 gtccagcgga gatcctggca aagggcccat cacgcgctac gtaatagagg ccaggccttc
     7201 agatgagggg ctctgggaca ttctcatcaa ggatatcccc aaggaggtga cttcctacac
     7261 cttcagcatg gacatcctga agccaggcgt aagctatgac ttccgggtca tcgcagtcaa
     7321 cgactacggc tttggcactc ccagcagccc ctcccagtct gtgccagccc agaaagccag
     7381 ccccttttat gaggaaggcg cgcctgacaa aactcacaca tgcccaccgt gcccagcacc
     7441 tgaactcctg gggggaccgt cagtcttcct cttcccccca aaacccaagg acaccctcat
     7501 gatctcccgg acccctgagg tcacatgcgt ggtggtggac gtgagccacg aagaccctga
     7561 ggtcaagttc aactggtacg tggacggcgt ggaggtgcat aatgccaaga caaagccgcg
     7621 ggaggagcag tacaacagca cgtaccgtgt ggtcagcgtc ctcaccgtcc tgcaccagga
     7681 ctggctgaat ggcaaggagt acaagtgcaa ggtctccaac aaagccctcc cagcccccat
     7741 cgagaaaacc atctccaaag ccaaagggca gccccgagaa ccacaggtgt acaccctgcc
     7801 cccatcccgg gatgagctga ccaagaacca ggtcagcctg acctgcctgg tcaaaggctt
     7861 ctatcccagc gacatcgccg tggagtggga gagcaatggg cagccggaga acaactacaa
     7921 gaccacgcct cccgtgctgg actccgacgg ctccttcttc ctctacagca agctcaccgt
     7981 ggacaagagc aggtggcagc aggggaacgt cttctcatgc tccgtgatgc atgaggctct
     8041 gcacaaccac tacacacaga agagcctctc cctgtctccg ggtaaaggcg gccatcatca
     8101 ccatcaccat taatctagag gatcttatta aagcagaact tgtttattgc agcttataat
     8161 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     8221 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggtc gactctagct
     8281 agactcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     8341 ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     8401 aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     8461 ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     8521 gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     8581 cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     8641 gggaagcgtg gcgctttctc atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     8701 tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     8761 cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     8821 cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     8881 gtggcctaac tacggctaca ctagaagaac agtatttggt atctgcgctc tgctgaagcc
     8941 agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     9001 cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     9061 tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     9121 tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     9181 ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     9241 cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     9301 cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     9361 accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     9421 ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     9481 ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     9541 tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     9601 acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     9661 tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     9721 actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     9781 ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     9841 aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     9901 ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     9961 cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
    10021 aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
    10081 actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
    10141 cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
    10201 ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
    10261 taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt gatgacggtg aaaacctctg
    10321 acacatgcag ctcccggagg aatgtgtgtc agttagggtg tggaaagtcc ccaggctccc
    10381 caggcaggca gaagtatgca aagcatgcat ctcaattagt cagcaaccag gtgtggaaag
    10441 tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
    10501 atagtcccgc ccctaactcc gcccatcccg cccctaactc cgcccagttc cgcccattct
    10561 ccgccccatg gctgactaat tttttttatt tatgcagagg ccgaggccgc ctctgcctct
    10621 gagctattcc agaagtagtg aggaggcttt tttggaggcc taggcttttg caaaaagctc
    10681 ccgggagctt gtatatccat tttcgtcccg gagacggtca cagcttgtct gtaagcggat
    10741 gccgggagca gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg
    10801 cttaactatg cggcatcaga gcagattgta ctgagagtgc accatatgcg gtgtgaaata
    10861 ccgcacagat gcgtaaggag aaaataccgc atcaggaaat tgtaagcgtt aatattttgt
    10921 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
    10981 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
    11041 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
    11101 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
    11161 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
    11221 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
    11281 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
    11341 tacagggcgc gtccattcgc cattcaggct gcgcaactgt tgggaagggc gatcggtgcg
    11401 ggcctcttcg ctattacgcc agctggcgaa agggggatgt gctgcaaggc gattaagttg
    11461 ggtaacgcca gggttttccc agtcacgacg ttgtaaaacg acggccagtg aatt
//
