LOCUS       sequence-139899-         899 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  Soluble red fluorescent activator of Orai1 channels.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 899)
  AUTHORS   Dynes JL, Amcheslavsky A, Cahalan MD
  TITLE     Genetically targeted single-channel optical recording reveals 
            multiple Orai1 gating states and oscillations in calcium influx.
  JOURNAL   Proc Natl Acad Sci U S A. 2016 Jan 12;113(2):440-5. doi: 
            10.1073/pnas.1523410113. Epub 2015 Dec 28.
  PUBMED    26712003
REFERENCE   2  (bases 1 to 899)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; doi: 
            "10.1073/pnas.1523410113"; journalName: "Proc Natl Acad Sci U S A"; 
            date: "2016-01-12- 12"; volume: "113"; issue: "2"; pages: "440-5"
COMMENT     Sequence Label: mCherry-CAD
FEATURES             Location/Qualifiers
     source          1..899
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      38..47
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             44..751
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNF
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     primer_bind     complement(189..207)
                     /label=mCherry-R
                     /note="mCherry, reverse primer"
     primer_bind     457..476
                     /label=mCherry-F
                     /note="mCherry, forward primer"
ORIGIN
        1 gtttagtgaa ccgtcagatc cgctagcgct accggtcgcc accatggtga gcaagggcga
       61 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
      121 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
      181 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
      241 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
      301 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttcgagga
      361 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
      421 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
      481 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
      541 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
      601 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
      661 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
      721 ctccaccggc ggcatggacg agctgtacaa gtccggactc agatctcgag ctcaagcttc
      781 gaattcttat gctccagagg cccttcagaa gtggctgcag ctgacacatg aggtggaggt
      841 gcaatattac aacatcaaga agcaaaatgc tgagaagcag ctgctggtgg ccaaggagg
//
