LOCUS       sequence-139906-        9617 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the N-terminal extracellular region of the PlexinB1 
            protein following proteolytic cleavage (ie, short), C-terminally 
            fused to the Fc region of human IgG1 + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9617)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 9617)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Plxnb1(S)-Fc-His
FEATURES             Location/Qualifiers
     source          1..9617
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     regulatory      4038..4047
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     CDS             6197..6214
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    6240..6361
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(6277..6296)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     6331..6350
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(6399..6416)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(6570..7158)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(6650..6669)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(7329..8120,8121..8189))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     7952..7971
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(8190..8294)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8362..8380
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(8418..8440)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(8442..8462)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        8447..8777
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      8628..8763
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8690..8709
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     8914..8933
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(9000..9455)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(9137..9158)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     9349..9368
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     9581..9603
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     9595..9612
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     9596..9612
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattctcgag gatccgcggc cgcgccacca tgtctgtcct
     1621 cggcccagtt cttctccagg tgttctgggc cgggtgtgtc gtcaccctgc ggtcccctct
     1681 gccagctgct ttcactgcca atggcacaca tctacaacac ttggcaaggg accccaccac
     1741 aggtaccctc tatgtagggg ccaccaactt cctgttccag ttgagccctg ggctgcagct
     1801 ggaagccgtg gtgtccacgg gccctgtgaa tgacagccgg gattgcctgc cacctgtgat
     1861 acctgatgaa tgtccccaag cccagcctac taacaaccct aaccagctgc tcctggtgag
     1921 cccagaggct ctggtggtgt gtgggagcat acaccagggc atctgtgagc tacggagcct
     1981 gggacagatc aggcagctgc tgctacggcc agagcgacct ggggacaccc agtatgtggc
     2041 tgcaaatgac cctgcagtca gtacagtggg gctggtggcc cagggattgg taggggagcc
     2101 cctcctgttt gtggggcggg gatacaccag caggggtgta ggtggtggga ttcctcccat
     2161 tacaacccga gccctgcgac caccggaccc ccaagctgcc ttctcttatg aagaaacagc
     2221 caagttagca gtgggccgcc tgtccgagta cagccaccac ttcgtgagtg cctttgtacg
     2281 cggggccagt gcatacttcc tgttcttgcg gcgagacctg aaggcccctt ctagagcttt
     2341 ccgtgcctat gtgtctcgag tgtgccttca ggaccagcac tactactctt atgtggaatt
     2401 gcccctggcc tgccagggtg gtcgttacgg tcttatccag gctgcagctg tagccacgtc
     2461 caaggaggtg gcccgtgggg acgtactctt tgcagctttc tcctcagtgg ctcctcccac
     2521 tgtggattgg cccctgtcag catctactgg ggcatctgga acctctgtgc tctgtgcctt
     2581 ccccctggat gaggtagacc agcttgctaa ttacactcga gatgcctgtt atactcggga
     2641 aggccgtgct gagaacggga ccaaggttgc tgacattgca tacgatgtcc tttccgactg
     2701 tgcgcagcta ccagtggaca ccccggatgc ttttccatgt ggctctgacc acacacccag
     2761 tcccatggtc agctgtgtcc ctttggaagc cacgccaatt ctggagctac caggggttca
     2821 gctaacagct gtggctgtca ccatggagga tggacacact attgctttcc tgggtgacag
     2881 tcaaggacag ttgcataggg tctacttagg ccctggaaga agtgctgccc catattctaa
     2941 acagagcatc cagccggggt ctcctgtgaa cagagatctt acctttgatg gtacctttga
     3001 gcatctctat gtagcaactc agactactct tgtgaaggtt cctgtggctc cttgtgctca
     3061 gcatctggac tgtgactctt gccttgctca cagggaccct tattgcggat ggtgtgtgct
     3121 cctgggcagg tgtagtcgcc ggtcggagtg ctcaagggac cagggcccag agcagtggct
     3181 gtggagcttc cagccggaac tgggctgtct tcgagtggtg gccgtgagcc ctgccaatat
     3241 cagtcgggaa gagaggaggg aggttttctt gtcagtgcca ggcctgccat ctctctggcc
     3301 aggggagtca tatttctgct actttggaga ccaacagagt cctgctctac tgaccagttc
     3361 tggtgtgatg tgtccctccc cagaccccag tgaggctcca gtgctgcaga gaggagccga
     3421 ccatatctct gtgaacgtgg agctcaggtt tggtgccgtg gtgatcgcca gcacctccct
     3481 ctccttctat gactgcgtgg cagttactgc gtcttcccca tctgcaccgt gccgggcctg
     3541 tgtgagcagc cgctggggct gtaactggtg tgtgtggcag cagctgtgca cacacaaggc
     3601 ctcgtgtgac gctgggccta tggtggcaag ccaacagagc ccactccttc ccctaatccc
     3661 tcctgcaagg gatgaactca cccctttccc acccacagtc ccccaaacca cggtcactcc
     3721 tacccccaac agcttcccaa tagagcctag ggctccctcc acagcctcag atgtcctacc
     3781 tggggccaag ccttcccggc tcagcctctg gggcccatgg gcaggtcctg gccccatact
     3841 ttcccctact tccacagagt cacctcttca tgagaagccc cttcctcctg acccccctac
     3901 catacctgga accactgtcc ctgcccccac tggcttggga ccatcgacca cacctgagga
     3961 cctcttggcc tcctacccat tcccctcaga tgcagctgca gtgtcccctg cagagcctgg
     4021 ccctgaggct ctgccttcca tggtggctct ggaccagccc cctggcactg ttccagacac
     4081 tactttccca ggggcccctg gctccatgaa gcccgttctg gattggctca ccaaaggagg
     4141 cggcgagctg cccgaggcgg atgagtggat ggggggtgac acgcccgcct tctccacttc
     4201 cacactcctc tcaggtgatg gagactcagc agagcacgag ggccctcctg cccccctcat
     4261 cctcctgtcc agcctcgact accagtacga cacccccggg ctctgggagc tgggagaggt
     4321 gaatcagagg gtgagctcct gcccctgtgt ggagaccgtc cagggctcct tgctgatacc
     4381 ggtccatgtg gaacgcgaag tccagcttcg aggcaggaac ctgtggcttt tccaggatgg
     4441 cccgaggagc agcgagtgtg tgctggagct agggagtcgg gaggtggctg tggaggctca
     4501 ggtggagtgt gcgccgcctc cagatgtctg gtgccacatc aagtgccagc agcatcagtt
     4561 cagctatgaa gctttgaagc cagaactgca ggtggggctg ttcctgcgtt gggcaggcgg
     4621 tctgcgcgtg gacagtgccg atgggctgca tgtggtgttg tatgactgct ctgtgggaca
     4681 tggggactgc agccgctgcc aaactgccat gcctcagtac gactgtgtgt ggtgtgaggg
     4741 ggagcgtccg cgttgtgtgg cccgggaagc ctgtaatgaa gccgagactg tggccactca
     4801 gtgccccgca cctctcattc actcggtgga tccactgact ggacctatag atggaggcac
     4861 ccgtgtcact atcaggggct ccaacctggg ccaacatgtg caggatgtcc tggacatggt
     4921 cagagtggcc ggagttccct gcgctgtgga tgctggggag tatgatgtct ctagtagtct
     4981 tgtgtgcatc actggagcca gcggggagga ggtgactggc actgtggcag tggaggtgcc
     5041 tggaagagga cacggtgtct cagagttcag ctttgcctat caggatccaa aagtacactc
     5101 catcttccca gcccgtggcc ctagagctgg aggtacccgc cttaccctgc atggttctaa
     5161 gctcctgact ggacggctag aggacatccg tgtggtggtt ggagaccagc cttgccacct
     5221 gctcctggag cagcagtctg agcagctaca ctgtgagacc ggcccatacc ctgtgcctgc
     5281 tgaacttcca gtgactgtct tgtttggggc cactgagcgg aggcttcagc acggccaatt
     5341 caagtataca tcagacccca atgtcacctc agtgggcccc tccaagagct tcttcagcgg
     5401 aggacgtgag atatgggtcc gcggccagga tcttgatgtg gtacagaggc caagaatccg
     5461 agtgaccgtg gtcccaagac agcatggcca ggggcttgca ggcgcgcctg acaaaactca
     5521 cacatgccca ccgtgcccag cacctgaact cctgggggga ccgtcagtct tcctcttccc
     5581 cccaaaaccc aaggacaccc tcatgatctc ccggacccct gaggtcacat gcgtggtggt
     5641 ggacgtgagc cacgaagacc ctgaggtcaa gttcaactgg tacgtggacg gcgtggaggt
     5701 gcataatgcc aagacaaagc cgcgggagga gcagtacaac agcacgtacc gtgtggtcag
     5761 cgtcctcacc gtcctgcacc aggactggct gaatggcaag gagtacaagt gcaaggtctc
     5821 caacaaagcc ctcccagccc ccatcgagaa aaccatctcc aaagccaaag ggcagccccg
     5881 agaaccacag gtgtataccc tgcccccatc ccgggatgag ctgaccaaga accaggtcag
     5941 cctgacctgc ctggtcaaag gcttctatcc cagcgacatc gccgtggagt gggagagcaa
     6001 tgggcagccg gagaacaact acaagaccac gcctcccgtg ctggactccg acggctcctt
     6061 cttcctctac agcaagctca ccgtggacaa gagcaggtgg cagcagggga acgtcttctc
     6121 atgctccgtg atgcatgagg ctctgcacaa ccactacacg cagaagagcc tctccctgtc
     6181 tccgggtaaa ggcggccatc atcaccatca ccattaatct agagatctta ttaaagcaga
     6241 acttgtttat tgcagcttat aatggttaca aataaagcaa tagcatcaca aatttcacaa
     6301 ataaagcatt tttttcactg cattctagtt gtggtttgtc caaactcatc aatgtatctt
     6361 atcatgtctg gtcgactcta gctagactct tccgcttcct cgctcactga ctcgctgcgc
     6421 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
     6481 acagaatcag gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg
     6541 aaccgtaaaa aggccgcgtt gctggcgttt ttccataggc tccgcccccc tgacgagcat
     6601 cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag
     6661 gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga
     6721 tacctgtccg cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg
     6781 tatctcagtt cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt
     6841 cagcccgacc gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac
     6901 gacttatcgc cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc
     6961 ggtgctacag agttcttgaa gtggtggcct aactacggct acactagaag aacagtattt
     7021 ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc
     7081 ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc
     7141 agaaaaaaag gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg
     7201 aacgaaaact cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag
     7261 atccttttaa attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg
     7321 tctgacagtt accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt
     7381 tcatccatag ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca
     7441 tctggcccca gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca
     7501 gcaataaacc agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc
     7561 tccatccagt ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt
     7621 ttgcgcaacg ttgttgccat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg
     7681 gcttcattca gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc
     7741 aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg
     7801 ttatcactca tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga
     7861 tgcttttctg tgactggtga gtactcaacc aagtcattct gagaatagtg tatgcggcga
     7921 ccgagttgct cttgcccggc gtcaatacgg gataataccg cgccacatag cagaacttta
     7981 aaagtgctca tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg
     8041 ttgagatcca gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact
     8101 ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata
     8161 agggcgacac ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt
     8221 tatcagggtt attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa
     8281 ataggggttc cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt
     8341 atcatgacat taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc
     8401 ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg aggaatgtgt gtcagttagg
     8461 gtgtggaaag tccccaggct ccccaggcag gcagaagtat gcaaagcatg catctcaatt
     8521 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     8581 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     8641 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     8701 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     8761 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgtc ccggagacgg
     8821 tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg
     8881 gtgttggcgg gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag
     8941 tgcaccatat gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcagga
     9001 aattgtaagc gttaatattt tgttaaaatt cgcgttaaat ttttgttaaa tcagctcatt
     9061 ttttaaccaa taggccgaaa tcggcaaaat cccttataaa tcaaaagaat agaccgagat
     9121 agggttgagt gttgttccag tttggaacaa gagtccacta ttaaagaacg tggactccaa
     9181 cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca ctacgtgaac catcacccta
     9241 atcaagtttt ttggggtcga ggtgccgtaa agcactaaat cggaacccta aagggagccc
     9301 ccgatttaga gcttgacggg gaaagccggc gaacgtggcg agaaaggaag ggaagaaagc
     9361 gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc acgctgcgcg taaccaccac
     9421 acccgccgcg cttaatgcgc cgctacaggg cgcgtccatt cgccattcag gctgcgcaac
     9481 tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc gaaaggggga
     9541 tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg acgttgtaaa
     9601 acgacggcca gtgaatt
//
