LOCUS       sequence-139915-       11661 bp    DNA     circular SYN 15-DEC-2025
DEFINITION  Expresses the extracellular region of the Sdk1 protein (endogenous 
            signal peptide replaced with CD33 signal peptide), C-terminally 
            fused to the Fc region of human IgG1 + 6X histidine tag..
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11661)
  AUTHORS   Visser JJ, Cheng Y, Perry SC, Chastain AB, Parsa B, Masri SS, Ray 
            TA, Kay JN, Wojtowicz WM
  TITLE     An extracellular biochemical screen reveals that FLRTs and Unc5s 
            mediate neuronal subtype recognition in the retina.
  JOURNAL   Elife. 2015 Dec 3;4. pii: e08149. doi: 10.7554/eLife.08149.
  PUBMED    26633812
REFERENCE   2  (bases 1 to 11661)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Elife."; 
            date: "2015-12-3"; volume: "4. pii: e08149. doi"; pages: " 
            10.7554/eLife.08149"
COMMENT     Sequence Label: Sdk1-Fc-His
FEATURES             Location/Qualifiers
     source          1..11661
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     enhancer        25..408
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        409..612
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     primer_bind     563..583
                     /label=CMV-F
                     /note="Human CMV immediate early promoter, forward primer"
     primer_bind     609..633
                     /label=LNCX
                     /note="Human CMV promoter, forward primer"
     intron          748..1573
                     /label=CMV intron A
                     /note="human cytomegalovirus intron A (Chapman et al., 
                     1991)"
     CDS             8240..8257
                     /codon_start=1
                     /product="6xHis affinity tag"
                     /label=6xHis
                     /translation="HHHHHH"
     polyA_signal    8284..8405
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(8321..8340)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     8375..8394
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(8443..8460)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(8614..9202)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(8694..8713)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9373..10164,10165..10233))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     9996..10015
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10234..10338)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10406..10424
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10462..10484)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(10486..10506)
                     /label=pBABE 3'
                     /note="SV40 enhancer, reverse primer for pBABE vectors"
     promoter        10491..10821
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      10672..10807
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     10734..10753
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     primer_bind     10958..10977
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     rep_origin      complement(11044..11499)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(11181..11202)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     11393..11412
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     11625..11647
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     11639..11656
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     11640..11656
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 gtcgaggtct cgacggtatc gattgacatt gattattgac tagctagtta ttaatagtaa
       61 tcaattacgg ggtcattagt tcatagccca tatatggagt tccgcgttac ataacttacg
      121 gtaaatggcc cgcctggctg accgcccaac gacccccgcc cattgacgtc aataatgacg
      181 tatgttccca tagtaacgcc aatagggact ttccattgac gtcaatgggt ggagtattta
      241 cggtaaactg cccacttggc agtacatcaa gtgtatcata tgccaagtcc gccccctatt
      301 gacgtcaatg acggtaaatg gcccgcctgg cattatgccc agtacatgac cttacgggac
      361 tttcctactt ggcagtacat ctacgtatta gtcatcgcta ttaccatggt gatgcggttt
      421 tggcagtaca ccaatgggcg tggatagcgg tttgactcac ggggatttcc aagtctccac
      481 cccattgacg tcaatgggag tttgttttgg caccaaaatc aacgggactt tccaaaatgt
      541 cgtaataacc ccgccccgtt gacgcaaatg ggcggtaggc gtgtacggtg ggaggtctat
      601 ataagcagag ctcgtttagt gaaccgtcag atcgcctgga gacgccatcc acgctgtttt
      661 gacctccata gaagacaccg ggaccgatcc agcctccgcg gccgggaacg gtgcattgga
      721 acgcggattc cccgtgccaa gagtgacgta agtaccgcct atagactcta taggcacacc
      781 cctttggctc ttatgcatgc tatactgttt ttggcttggg gcctatacac ccccgcttcc
      841 atatgctata ggtgatggta tagcttagcc tataggtgtg ggttattgac cattattgac
      901 cactccccta ttggtgacga tactttccat tactaatcca taacatggct ctttgccaca
      961 actatctcta ttggctatat gccaatactc tgtccttcag agactgacac ggactctgta
     1021 tttttacagg atggggtccc atttattatt tacaaattca catatacaac aacgccgtcc
     1081 cccgtgcccg cagtttttat taaacatagc gtgggatctc cacgcgaatc tcgggtacgt
     1141 gttccggaca tgggctcttc tccggtagcg gcggagcttc cacatccgag ccctggtccc
     1201 atgcctccag cggctcatgg tcgctcggca gctccttgct cctaacagtg gaggccagac
     1261 ttaggcacag cacaatgccc accaccacca gtgtgccgca caaggccgtg gcggtagggt
     1321 atgtgtctga aaatgagctc ggagattggg ctcgcaccgc tgacgcagat ggaagactta
     1381 aggcagcggc agaagaagat gcaggcagct gagttgttgt attctgataa gagtcagagg
     1441 taactcccgt tgcggtgctg ttaacggtgg agggcagtgt agtctgagca gtactcgttg
     1501 ctgccgcgcg cgccaccaga cataatagct gacagactaa cagactgttc ctttccatgg
     1561 gtcttttctg cagaagcttg aattcgccac catgcccctg ctgctgctgc tgcccctgct
     1621 gtgggccggc gcggccgctg gacctggaag gcctaggagg agcaggaccg gtcaccactg
     1681 cgaccccgag tgcccagggc tgcgcgcagc gcccaggacc cccgggccgg gcgcgggccg
     1741 gcgggcggca aagttgaggc cgggccgcgg gtggtgggcg ctgctgttgc tccagctgca
     1801 cttgctgcgg gcgctggcgc aagatgacgt tgcaccgtat ttcaaaacgg agccgggcct
     1861 gccacagatc cacctggaag ggaaccgcct ggtgctcacc tgcctggctg aagggagctg
     1921 gcctctggaa ttcaagtgga tacggaacga cagtgagctt accacctaca gcagcgaata
     1981 taagtatatc atcccctctt tgcagaagct ggacgccggg ttttaccgct gcgtggtgcg
     2041 gaacaggatg ggagctctct tgcagagaaa atcagaaatt caagtagcat atatggggaa
     2101 cttcatggat acagaccaga ggaaaacagt ttctcaaggg catgcggcac ttctcaactt
     2161 actgcctatc gtcagctgcc cccaacctca agtgacgtgg tttagagaag gacacaagat
     2221 tattcccagc agcagaatag ccatcacatt ggagaatcag ctggtgatcc ttgctacaac
     2281 agcgagtgat gctggggctt actacgtgca ggctgtgaat gagaagaacg gagagaacaa
     2341 gacaagcccg ttcatccacc tgagcgtagc acgagatact ggtacccacg aggccatggc
     2401 cccaatcatc gtggttgccc caggcaacag aagtgtggta gcagggtcca gtgagaccac
     2461 cttggagtgc atagccaatg ccaggcccgt ggaggagctg agtgtgcact ggaagagaaa
     2521 cggagttcga ctcacaagtg gactacacag ctacgggagg cgccttacca tcaccaatcc
     2581 gacatcagca gacaccggga tgtacgtgtg cgaggcgaca ctccggggga gcacatttga
     2641 gccagccagg gccagagcct tcctttccat catagagcca ccatatttca ctgctgagcc
     2701 agagagtcgg attttagggg aagtggaaga aactatggac atcccatgtc gagccatggg
     2761 ggttcctctt cccactctcc agtggtacaa agatgctgtt cccctcagca aactccagaa
     2821 ccctaggtat aaagttctcc ccagcggagg cctccacatt cagaagttga gtccagaaga
     2881 ctctggaatc ttccagtgct ttgccagtaa cgaaggagga gaggtccaga ctcacaccta
     2941 cctggatgtg accaatattg ctcctgcctt cacccagcgt ccagtggaca ccacggttac
     3001 tgacgggatg acggctgtcc tgaggtgtga ggtgtccgga gctcccaaac ccgccatcac
     3061 atggaagaga gggaaccaca ttctggccag tggctctgtc cggattccta ggttcatgct
     3121 gctggaatct ggggggctga gaatagcccc cgtcttcatc caggatgctg gcaactacac
     3181 ctgctatgca gccaacacgg aggcttctgt gaacgcctcc gccatgctca ccgtgtggaa
     3241 tcgaacgtcc atcgttcacc ctccagaaga ccgcgtggtg attaagggga cgacggccac
     3301 cctgtgctgc ggagctaccc acgaccctcg gacctctctc cgctacgttt ggaaaaagga
     3361 caacgtggtc atcacagcgt ctagcagctc cagaattgtg gtggaaaagg atgggtcgct
     3421 tgtcattagc cagacgtggt caggggacat tggtgactac acctgtgaaa tcatatccga
     3481 gggagggagt gactcccgga cggcacggct ggaagtgatt gagctgcctc acccacctca
     3541 aaacctgctg gccagcttga gtccagcacg cagccactcg gtgacactat cttgggttcg
     3601 gccctttgat ggaaacagcc cggtccttta ctacattgtg caagtatctg aaaacaactc
     3661 tccatggaaa gtgcatttgt caaatgttgg cccggagatg acaggcgtca ctgtgagtgg
     3721 cttgactcca gcccggacct atcagttcag ggtgtgcgct gtgaatcaag tgggcaaggg
     3781 ccagtacagc acagagacaa gcagattgat gttacccgaa gaaccaccga gtgctccccc
     3841 aaaaaacatt gtggccagtg ggagaaccaa ccagtccatc atggtacagt ggcagccacc
     3901 acccgaaaca gagcacaatg gggtgcttcg gggctacatc ctcaggtacc gcctggctgg
     3961 cctgcctggg gaacaccagc agaggaacat cagcagccca gaggtgaact actgcctggt
     4021 caccgacctc atcatctgga cccagtatga gatccaggtg gctgcctaca acggggcagg
     4081 cctgggtgtc ttcagcaggg cagtgaccga gtacacctta caaggagtgc ccactgcacc
     4141 tccccagaac gtgcaggcag aggctgtgaa ttccaccact gtgcacttcc tgtggaaccc
     4201 gccaccccag cagttcatca acggcatcaa ccagggatac aagctcctag catggccggc
     4261 agatgctcct gagactgtca ctgtagtcac tattgctcct gacttccatg gaatccatca
     4321 tgggtacata acaaacttga agaagttcac tgcctacttc acatccgtcc tgtgttttac
     4381 caccccgggg gatgggcctc caagttcacc tcagctcgtg tggacccatg aggacaagcc
     4441 gggagctgtg gggcacctga gcttcacgga gattttggac acctctctca aggtcagctg
     4501 gcaggagccc ctggagagaa acggcatcat tatgggctat cagatttcct gggaggtgta
     4561 tgggaggaat gactctcgac tcacgcacac cctcaacagc acgatgcacg agtataagat
     4621 ccaaggactg tcatcactca ccacctatac catcgatgtg gctgccttga ccgccgcagg
     4681 cgtgggcgtg accacatcat ccaccatctc ttcaggtgta cccccagatc ttcctggcgc
     4741 tccatccaat ctggtcattt ccaacatcag ccctcgctca gccacactcc agttccgacc
     4801 tggctatgat gggaagacgg ccatctgcag gtggattgtg gaggggcagg ttggtgccat
     4861 cggggacgag gaggagtggg ttaccctcta tgaggaggag aatgagcccg atgcccagat
     4921 gttggagata ccaaacctca caccctacac acactacagg tttcgaatga ggcaagtgaa
     4981 catcgtgggt cccagcccct tcagccagtc atccagggtc atccagaccc tacaggcccc
     5041 acctgatgtg gctccaacca gcctcactgt ccgaacagcc agtgagacca gcctgaggct
     5101 acgctgggtg cctctgcctg attcccagta caatgggaac cctgagtctg tgggctacag
     5161 ggtgaagtat tggcgttctg accaaccatc gtcagctctg gcccaagttg tcagtgaccg
     5221 tctggagagg gagctgacca tcgaggaact ggaggagtgg acagagtacg agttgcggat
     5281 gcaggccttc aacgccattg gggctgggcc ctggagtgag ctggtgcggg gccgcacgcg
     5341 ggagtcagtt ccttcagcag ctcctgaaaa tgtgtctgca gaggctgtca gctccaccca
     5401 gatcctactg acttgggctt cagttcctga gcaggaccag aatgggctca tcctaggcta
     5461 caaggttcta tactgtgcca aagacctgga cccagagccc agaagccacg tggtacgagg
     5521 gaaccacacg cagtctgcgc tgctggctgg cctgcgaaag ttcgtggtct atgagctcca
     5581 agtgctggcg ttcacacgga tcgggaatgg ggtccccagc tcacctctca tcctggagcg
     5641 caccaaagat gacaccccag gtccccccgt gcggctggtg ttccccgaag taagacttac
     5701 tgcagtgcgg attgtgtggc agcccccaga ggagcccaat ggtgtgattc tggggtacca
     5761 gatcgcctac cgcctggcca gcggtagtcc tcacaccttc accactgttg aggtgggtgc
     5821 cacggtgcgg caattcacag ccacagagct ggccccggag tctgcctaca tcttcagact
     5881 gtctgctaag acgagacagg gttggggtga gcccctggag gccacagtta tcactactga
     5941 gaagagagag aggccagcgc cccccagaga gctcctggta ccccaggctg aagtgacagc
     6001 tcgtagcctc aggctccagt gggtccctgg cagtgatggg gcctctccga tccggtactt
     6061 cacggtgcag gtgcgagagc tgccaggtgg agagtggcag acctactcct catctatcag
     6121 tcatgaggcc acagcctgtg ctgttgagag gctgaggcct ttcacctcct acaagctgcg
     6181 cctgaaggcc accaatgaca ttggggacag tgacttcagt gcagaaacag aggctgtgac
     6241 cactttgcaa gatgttccag gagagccacc agggtctgtc tcagccacac cgcacaccac
     6301 gtcctccgtt ctgatccagt ggcagcctcc ccgggatgag agcctgaacg gtcttctgca
     6361 aggctacagg atctactacc gtgagctgga gtctgagaca ggcatgagcc ctgagcccaa
     6421 gacactcaag agcccctctg ccttacgtgc tgaactcacc gctcaaagca gcttcaagac
     6481 cgtgaacagc agctcctcgt taaccaccta tgaattaaca catctgaaga agtaccggcg
     6541 ctatgaagtc atcatgactg cttataacat cattggtgag agcccagcca gtgtaccagt
     6601 ggaagtcttc gttggtgagg ctgccccagc gatggcccca cagaatgtcc aggtgacccc
     6661 actcacggcc agccagctgg aggtcacatg ggacccaccg cccccagaga gccagaatgg
     6721 gaacatccag ggttataagg tttactactg ggaggcagac agtcgcaatg agacagagaa
     6781 aatgaaggtc ctcttcctcc ccgagcctgt ggtgaagatc aaggatctca ccagccacac
     6841 aaagtacctg atcagcatct cagccttcaa tgctgctggt gatgggccca agagtgaccc
     6901 gtgccaagga cgcacacatc aggcggctcc agggcccccc agcttcttgg cattctcaga
     6961 aataacttct accacactca acgtatcctg gggagagcca tcagctgcca acggcatcct
     7021 acagggctat cgagtggtgt atgagccctt agcaccagtg caaggcgtga gcaaggtggt
     7081 gaccgtggac gtgaaaggca actggcaacg ttggctgaag gtgcgggacc tcaccaaggg
     7141 agtgacctac ttcttccgtg tccaggcgcg gaccatcgcc tacgggccgg agctccaagc
     7201 caatgtcact gcagggccag ccgaggggtc cccaggctca ccaagaaatg tccttgtcac
     7261 caaatctgcc tctgagctga cccttcagtg gacagaggga aatgctggta ccacacccac
     7321 tacaggctac gtcatagaag ccaggccatc agatgaaggc ttatgggaca tgtttgcaaa
     7381 ggacattccc aggagtgcta catcatacac tgtggacctg gataagttgc ggcaaggggt
     7441 gacctacgag ttccgggtgg tggctgtgaa caaggcaggc tttggggagc ccagccgccc
     7501 ttccattgca gtgtcagcac aagctgaagc cccgttctac gaggagacta gtgacaaaac
     7561 tcacacatgc ccaccgtgcc cagcacctga actcctgggg ggaccgtcag tcttcctctt
     7621 ccccccaaaa cccaaggaca ccctcatgat ctcccggacc cctgaggtca catgcgtggt
     7681 ggtggacgtg agccacgaag accctgaggt caagttcaac tggtacgtgg acggcgtgga
     7741 ggtgcataat gccaagacaa agccgcggga ggagcagtac aacagcacgt accgtgtggt
     7801 cagcgtcctc accgtcctgc accaggactg gctgaatggc aaggagtaca agtgcaaggt
     7861 ctccaacaaa gccctcccag cccccatcga gaaaaccatc tccaaagcca aagggcagcc
     7921 ccgagaacca caggtgtata ccctgccccc atcccgggat gagctgacca agaaccaggt
     7981 cagcctgacc tgcctggtca aaggcttcta tcccagcgac atcgccgtgg agtgggagag
     8041 caatgggcag ccggagaaca actacaagac cacgcctccc gtgctggact ccgacggctc
     8101 cttcttcctc tacagcaagc tcaccgtgga caagagcagg tggcagcagg ggaacgtctt
     8161 ctcatgctcc gtgatgcatg aggctctgca caaccactac acgcagaaga gcctctccct
     8221 gtctccgggt aaaggcggcc atcatcacca tcaccattaa tctagaggat cttattaaag
     8281 cagaacttgt ttattgcagc ttataatggt tacaaataaa gcaatagcat cacaaatttc
     8341 acaaataaag catttttttc actgcattct agttgtggtt tgtccaaact catcaatgta
     8401 tcttatcatg tctggtcgac tctagctaga ctcttccgct tcctcgctca ctgactcgct
     8461 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
     8521 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
     8581 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
     8641 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
     8701 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
     8761 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
     8821 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
     8881 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
     8941 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
     9001 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaagaacagt
     9061 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
     9121 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
     9181 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     9241 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     9301 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     9361 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     9421 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     9481 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     9541 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     9601 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     9661 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     9721 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     9781 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     9841 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     9901 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     9961 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
    10021 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
    10081 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
    10141 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
    10201 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
    10261 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
    10321 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat
    10381 tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg
    10441 tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggaggaat gtgtgtcagt
    10501 tagggtgtgg aaagtcccca ggctccccag gcaggcagaa gtatgcaaag catgcatctc
    10561 aattagtcag caaccaggtg tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa
    10621 agcatgcatc tcaattagtc agcaaccata gtcccgcccc taactccgcc catcccgccc
    10681 ctaactccgc ccagttccgc ccattctccg ccccatggct gactaatttt ttttatttat
    10741 gcagaggccg aggccgcctc tgcctctgag ctattccaga agtagtgagg aggctttttt
    10801 ggaggcctag gcttttgcaa aaagctcccg ggagcttgta tatccatttt cgtcccggag
    10861 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
    10921 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
    10981 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
    11041 aggaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt taaatcagct
    11101 cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa gaatagaccg
    11161 agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag aacgtggact
    11221 ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt gaaccatcac
    11281 cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac cctaaaggga
    11341 gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga
    11401 aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca
    11461 ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc cattcgccat tcaggctgcg
    11521 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
    11581 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
    11641 taaaacgacg gccagtgaat t
//
