LOCUS       sequence-139919-         760 bp    DNA     linear   UNA 15-DEC-2025
DEFINITION  promoterless Tol2 vector with mCherry tag upstream of and in frame 
            with attR1/attR2 cassette and late SV40 polyadenylation signal.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      natural DNA sequence
  ORGANISM  unspecified
REFERENCE   1  (bases 1 to 760)
  AUTHORS   Villefranc JA, Amigo J, Lawson ND
  TITLE     Gateway compatible vectors for analysis of gene function in the 
            zebrafish.
  JOURNAL   Dev Dyn. 2007 Nov . 236(11):3077-87.
  PUBMED    17948311
REFERENCE   2  (bases 1 to 760)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Dec 15, 2025 from SnapGene Server 8.0.1
            https://www.snapgene.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Dev Dyn. 
            2007 Nov . 236(11):3077-87."
COMMENT     Sequence Label: 366 pTol2-CherryDest
FEATURES             Location/Qualifiers
     source          1..760
                     /mol_type="genomic DNA"
                     /organism="unspecified"
     regulatory      98..107
                     /label=Kozak sequence
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
                     /regulatory_class="other"
     misc_feature    join(509..671,672..683)
                     /label=Tol2 right end
                     /note="right end of the Tol2 transposon from the medaka 
                     fish Oryzias latipes (Kawakami, 2007)"
ORIGIN
        1 atctcgaact cgcggccgtt cacggagccc tccatgtgca ccttgaagcg catgaactcc
       61 ttgatgatgg ccatgttgtc ctcctcgccc ttgctcacca tggtggcgct agccttgaat
      121 tcgaatcgat gctagcgata tcggcccatc tggcctgtgt ttcagacacc agggagtctc
      181 tgctcacgtt tcctgctatt tgcagcctct ctatcaagac taatacacct cttcccgcat
      241 cggctgcctg tgagaggctt ttcagcactg caggattgct tttcagcccc aaaagagcta
      301 ggcttgacac taacaatttt gagaatcagc ttctactgaa gttaaatctg aggttttaca
      361 actttgagta gcgtgtactg gcattagatt gtctgtctta tagtttgata attaaataca
      421 aacagttcta aagcaggata aaaccttgta tgcatttcat ttaatgtttt ttgagattaa
      481 aagcttaaac aagaatctct agttttcttt cttgctttta cttttacttc cttaatactc
      541 aagtacaatt ttaatggagt acttttttac ttttactcaa gtaagattct agccagatac
      601 ttttactttt aattgagtaa aattttccct aagtacttgt actttcactt gagtaaaatt
      661 tttgagtact ttttacacct ctgtcaagaa ctcctggaca aacctctgac ctgtgtggaa
      721 cagagtggat atgggtgtct gaacagatat tcacgtcttt
//
